Theories to support method development in comprehensive two-dimensional liquid chromatography - A review

	Theories to support method development in comprehensive two-dimensional liquid chromatography - A review
	Bedani, Filippo 1,2; Schoenmakers, Peter J.2; Janssen, Hans-Gerd 2,3
刊名	journal of separation science
	2012-07-01
卷号	35 期号:14 页码:1697-1711
关键词	Degree of orthogonality First-dimension undersampling LC LC method development LC LC optimization On-line comprehensive two-dimensional liquid chromatography
英文摘要	on-line comprehensive two-dimensional liquid chromatography techniques promise to resolve samples that current one-dimensional liquid chromatography methods cannot adequately deal with. to make full use of the potential of two-dimensional liquid chromatography, optimization is required. optimization of two-dimensional liquid chromatography is a relatively new yet important research topic the aim of which is to predict combinations of stationary and mobile phases, column formats, and chromatographic conditions that maximize resolving power and minimize analysis time. in on-line two-dimensional liquid chromatography, dilution-related issues play also an important role and these should be taken into account when developing optimization strategies. in this work, state-of-the-art strategies that support method development for on-line two-dimensional liquid chromatography through a rigorous choice of chromatographic parameters are critically reviewed. the final aim is to provide practitioners with a clear understanding of which aspects can be optimized using current on-line two-dimensional liquid chromatography strategies (and which ones cannot). in two-dimensional liquid chromatography, maximizing resolving power for a given analysis time and dilution requires optimizing efficiency, selectivity and retention. while great strides forward have been made in the optimization of efficiency-related issues, considerable effort needs still to be made in terms of (1) developing models that can predict the retention factors that given stationary/mobile phase systems can provide and (2) using this information for choosing the two ones that maximize two-dimensional liquid chromatography orthogonality. because of this limitation, in two-dimensional liquid chromatography, this aspect is typically dealt with a posteriori through examining chromatograms. this review clearly shows that important progress in the optimization of on-line two-dimensional liquid chromatography has recently been made.
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	hydrophobic-subtraction model ; single complex chromatograms ; reversed-phase separations ; peak-capacity ; high-pressure ; 2nd-dimension gradient ; system selectivity ; information-theory ; statistical-method ; stationary phases
收录类别	SCI
语种	英语
WOS记录号	WOS:000306493700001
公开日期	2015-11-17
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/142918]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Amsterdam, Vant Hoff Inst Mol Sci, Analyt Chem Grp, Amsterdam, Netherlands 3.Unilever Res Labs, Adv Measurement & Data Modelling, Vlaardingen, Netherlands
推荐引用方式 GB/T 7714	Bedani, Filippo,Schoenmakers, Peter J.,Janssen, Hans-Gerd. Theories to support method development in comprehensive two-dimensional liquid chromatography - A review[J]. journal of separation science,2012,35(14):1697-1711.
APA	Bedani, Filippo,Schoenmakers, Peter J.,&Janssen, Hans-Gerd.(2012).Theories to support method development in comprehensive two-dimensional liquid chromatography - A review.journal of separation science,35(14),1697-1711.
MLA	Bedani, Filippo,et al."Theories to support method development in comprehensive two-dimensional liquid chromatography - A review".journal of separation science 35.14(2012):1697-1711.