From proteomic analysis to clinical significance - Overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis

	From proteomic analysis to clinical significance - Overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis
	Ding, SJ; Li, Y; Tan, YX; Jiang, MR; Tian, B; Liu, YK; Shao, XX; Ye, SL; Wu, JR; Zeng, R
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2004
卷号	3 期号:1 页码:73-81
通讯作者	Xia, QC (reprint author), Shanghai Inst Biochem & Cell Biol, Yueyang Rd, Shanghai 200031, Peoples R China.,hywangk@online.sh.cn ; zytang@scrap.stc.sh.cn ; xiaqc@sibs.ac.cn
英文摘要	To better understand the mechanism underlying the hepatocellular carcinoma (HCC) metastasis and to search potential markers for HCC prognosis, differential proteomic analysis on two well-established HCC cell strains with high and low metastatic potentials, MHCC97-H and MHCC97-L, was conducted using two-dimensional gel electrophoresis followed by matrix-assisted laser desorption/time-of-flight mass spectrometry. Cytokeratin 19 (CK19) was identified and found to be overexpressed in MHCC97-H as compared with MHCC97-L. This result was further confirmed by two-dimensional Western blot analysis and immunofluorescence assay. Furthermore, one-dimensional Western blot analysis showed consistently increased CK19 expression in progressively more metastatic cells. Immunohistochemical study on 102 human HCC specimens revealed that more patients in the CK19-positive group had overt intrahepatic metastases ( satellite nodules, p < 0.05; vascular tumor emboli, p < 0.001; tumor node metastatis staging, p < 0.001). CK19 fragment CYFRA 21-1 levels measured in sera from nude mice model of human HCC metastasis with radioimmunoassay increased in parallel with tumor progression and rose remarkably when pulmonary metastases occurred. The results demonstrated that overexpression of CK19 in HCC cells is related to metastatic behavior. Serum CK19 level might reflect the pathological progression in some HCC and may be a useful marker for predicting tumor metastasis and a therapeutic target for the treatment of HCC patients with metastases.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemical Research Methods
关键词[WOS]	CELL-LINE ; MASS-SPECTROMETRY ; KERATIN FILAMENTS ; SERUM CYFRA-21-1 ; HUMAN HEPATOMA ; GROWTH-FACTOR ; LUNG-CANCER ; E-CADHERIN ; EXPRESSION ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000188432800007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/2028]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ding, SJ,Li, Y,Tan, YX,et al. From proteomic analysis to clinical significance - Overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis[J]. MOLECULAR & CELLULAR PROTEOMICS,2004,3(1):73-81.
APA	Ding, SJ.,Li, Y.,Tan, YX.,Jiang, MR.,Tian, B.,...&Xia, QC.(2004).From proteomic analysis to clinical significance - Overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis.MOLECULAR & CELLULAR PROTEOMICS,3(1),73-81.
MLA	Ding, SJ,et al."From proteomic analysis to clinical significance - Overexpression of cytokeratin 19 correlates with hepatocellular carcinoma metastasis".MOLECULAR & CELLULAR PROTEOMICS 3.1(2004):73-81.