The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity

	The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity
	Zhang, DH; Zhou, B; Huang, Y; Xu, LX; Zhou, JQ
刊名	NUCLEIC ACIDS RESEARCH
	2006
卷号	34 期号:5 页码:1393-1404
通讯作者	Zhou, JQ (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol,Grad Sch, State Key Lab Mol Biol,Max Planck Jr Res Grp, Shanghai 200031, Peoples R China.,jqzhou@sibs.ac.cn
英文摘要	Telomeres, the protein-DNA complexes at the ends of eukaryotic chromosomes, are essential for chromosome stability, and their maintenance is achieved by the specialized reverse transcriptase activity of telomerase or the homologous recombination pathway in most eukaryotes. Here, we identified a human helicase, hPif1 that inhibits telomerase activity. The primary sequence and biochemical analysis suggest that hPif1 is a potential homologue of Escherichia coli RecD, an ATP-dependent 5' to 3' DNA helicase. Ectopic expression of wild-type, but not the ATPase/helicase-deficient hPif1, causes telomere shortening in HT1080 cells. hPif1 reduces telomerase processivity and unwinds DNA/RNA duplex in vitro. hPif1 preferentially binds telomeric DNA in vitro and in vivo. We propose that the mechanism of hPif1's inhibition on telomerase involves unwinding of the DNA/RNA duplex formed by telomerase RNA and telomeric DNA, and RecD homologues in eukaryotes may have evolved gaining additional functions.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	REPLICATION FORK PROGRESSION ; NORMAL HUMAN-CELLS ; DNA HELICASE ; HUMAN FIBROBLASTS ; RECBCD ENZYME ; LIFE-SPAN ; IN-VITRO ; T-LOOP ; PROTEIN ; CANCER
收录类别	SCI
语种	英语
WOS记录号	WOS:000236432900019
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1721]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, DH,Zhou, B,Huang, Y,et al. The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity[J]. NUCLEIC ACIDS RESEARCH,2006,34(5):1393-1404.
APA	Zhang, DH,Zhou, B,Huang, Y,Xu, LX,&Zhou, JQ.(2006).The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity.NUCLEIC ACIDS RESEARCH,34(5),1393-1404.
MLA	Zhang, DH,et al."The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity".NUCLEIC ACIDS RESEARCH 34.5(2006):1393-1404.