Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production

	Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production
	Liu, YL; Jiang, S; Ke, ZM; Wu, HS; Chi, CW; Guo, ZY
刊名	CARBOHYDRATE RESEARCH
	2009
卷号	344 期号:6 页码:815-819
关键词	Chitosanase Recombinant expression Chitosan oligosaccharides
通讯作者	Guo, ZY (reprint author), Tongji Univ, Inst Prot Res, Coll Life Sci & Technol, 1239 Siping Rd, Shanghai 200092, Peoples R China.,zhan-yun.guo@mail.tongji.edu.cn
英文摘要	Recently, considerable attention has been focused on chitosan oligosaccharides (COSs) due to their various biological activities. COSs can be prepared by enzymatic degradation of chitosan, which is the deacetylation product Of Chitin, one of the most abundant biopolymers in nature. In the current study, we recombinantly expressed a chitosanase and used it for COS preparation. A bacillus-derived GH8 family chitosanase with a 6xHis tag fused at its N-terminal was expressed in the Escherichia coli strain BL21(DE3) as a Soluble and active form. Its expression level could be as high as 500 mg/L. Enzymatic activity could reach approximately 140,000 U/L under our assay conditions. The recombinant chitosanase could be purified essentially to homogeneity by immobilized metal-ion affinity chromatography. The enzyme Could efficiently convert chitosan into monomer-free COS: I g of enzyme could hydrolyze about 100 kg of chitosan. Our present work has provided a cheap chitosanase for large-scale COS production in industry. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题	Biochemistry & Molecular Biology; Chemistry
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Applied ; Chemistry, Organic
关键词[WOS]	PURIFICATION ; CHITOOLIGOSACCHARIDES ; CLONING ; CHITIN ; ACCUMULATION ; HYDROLYSIS ; PEPSIN ; GROWTH ; ENZYME ; YIELD
收录类别	SCI
语种	英语
WOS记录号	WOS:000265724700014
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/1211]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Liu, YL,Jiang, S,Ke, ZM,et al. Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production[J]. CARBOHYDRATE RESEARCH,2009,344(6):815-819.
APA	Liu, YL,Jiang, S,Ke, ZM,Wu, HS,Chi, CW,&Guo, ZY.(2009).Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production.CARBOHYDRATE RESEARCH,344(6),815-819.
MLA	Liu, YL,et al."Recombinant expression of a chitosanase and its application in chitosan oligosaccharide production".CARBOHYDRATE RESEARCH 344.6(2009):815-819.