Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3

	Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3
	Zhou, YZ; Li, SS; Huang, QP; Xie, LL; Zhu, XL
刊名	JOURNAL OF MOLECULAR CELL BIOLOGY
	2013
卷号	5 期号:4 页码:239-249
关键词	differentiation migration stem cells Nanog
通讯作者	Zhu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,xlzhu@sibcb.ac.cn
英文摘要	Nanog, Sox2, and Oct4 are key transcription factors critical for the pluripotency and self-renewal of embryonic stem (ES) cells. Their downregulations lead to differentiation, accompanied with changes in cell motility. Whether these factors impact cell motility directly, however, is not clear. Here we addressed this question by initially assessing their effect in non-stem cells. We found that the ectopic expression of Nanog, Sox2, or Oct4 markedly inhibited ECV304 cell migration. Detailed examinations revealed that Nanog induced disorganizations of the actin cytoskeleton and peripheral localizations of focal adhesions. These effects required its DNA-binding domain and are thus transcription dependent. Furthermore, thymosin 4 and Rnd3 were identified as its downstream targets. Their depletions in ECV304 cells by RNAi phenocopied the ectopic expression of Nanog in both cell motility and actin organization, whereas their ectopic expressions rescued the migration defect of Nanog overexpression. Both proteins were upregulated during mouse ES cell differentiation. Their levels in the pluripotent mouse P19 cells also increased upon Nanog ablation, coincident with an increase in cell motility. Moreover, persistent expression of Nanog in zebrafish embryos suppressed gastrulation and cell migration. These results indeed suggest a dual role of certain transcription factors in the orchestration of differentiation and motility.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	EMBRYONIC STEM-CELLS ; RNA INTERFERENCE ; DIFFERENTIATION ; PLURIPOTENCY ; ZEBRAFISH ; MOTILITY ; OCT4 ; SOX2 ; IDENTIFICATION ; GASTRULATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000322914000004
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/372]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhou, YZ,Li, SS,Huang, QP,et al. Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2013,5(4):239-249.
APA	Zhou, YZ,Li, SS,Huang, QP,Xie, LL,&Zhu, XL.(2013).Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3.JOURNAL OF MOLECULAR CELL BIOLOGY,5(4),239-249.
MLA	Zhou, YZ,et al."Nanog suppresses cell migration by downregulating thymosin beta 4 and Rnd3".JOURNAL OF MOLECULAR CELL BIOLOGY 5.4(2013):239-249.