A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18

	A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18
	Chi, BK; Wang, K; Du, YH; Gui, B; Chang, XY; Wang, LT; Fan, J; Chen, S; Wu, XD; Li, GH
刊名	NUCLEIC ACIDS RESEARCH
	2014
卷号	42 期号:11 页码:7305-7318
通讯作者	Cheng, H (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China.,ghli@dicp.ac.cn ; hcheng@sibcb.ac.cn
英文摘要	Viral RNA elements that facilitate mRNA export are useful tools for identifying cellular RNA export factors. Here we show that hepatitis B virus post-transcriptional element (PRE) is one such element, and using PRE several new cellular mRNA export factors were identified. We found that PRE drastically enhances the cytoplasmic accumulation of cDNA transcripts independent of any viral protein. Systematic deletion analysis revealed the existence of a 116 nt functional Sub-Element of PRE (SEP1). The RNP that forms on the SEP1 RNA was affinity purified, in which TREX components as well as several other proteins were identified. TREX components and the SEP1-associating protein ZC3H18 are required for SEP1-mediated mRNA export. Significantly, ZC3H18 directly binds to the SEP1 RNA, interacts with TREX and is required for stable association of TREX with the SEP1-containing mRNA. Requirements for SEP1-mediated mRNA export are similar to those for splicing-dependent mRNA export. Consistent with these similarities, several SEP1-interacting proteins, including ZC3H18, ARS2, Acinus and Brr2, are required for efficient nuclear export of polyA RNAs. Together, our data indicate that SEP1 enhances mRNA export by recruiting TREX via ZC3H18. The new mRNA export factors that we identified might be involved in cap- and splicing-dependent TREX recruitment to cellular mRNAs.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	POSTTRANSCRIPTIONAL REGULATORY ELEMENT ; CAP-BINDING COMPLEX ; PROTEOMIC ANALYSIS ; VIRUS TYPE-1 ; THO COMPLEX ; PROTEIN ; TRANSCRIPTION ; COMPONENTS ; REV ; POLYADENYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000338769400049
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/244]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Chi, BK,Wang, K,Du, YH,et al. A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18[J]. NUCLEIC ACIDS RESEARCH,2014,42(11):7305-7318.
APA	Chi, BK.,Wang, K.,Du, YH.,Gui, B.,Chang, XY.,...&Cheng, H.(2014).A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18.NUCLEIC ACIDS RESEARCH,42(11),7305-7318.
MLA	Chi, BK,et al."A Sub-Element in PRE enhances nuclear export of intronless mRNAs by recruiting the TREX complex via ZC3H18".NUCLEIC ACIDS RESEARCH 42.11(2014):7305-7318.