Identification and functional analysis of a novel IFN-like protein (CgIFNLP) in Crassostrea gigas

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	Identification and functional analysis of a novel IFN-like protein (CgIFNLP) in Crassostrea gigas
	Zhang, Ran 1; Liu, Rui 2; Wang, Weilin2,3 ; Xin, Lusheng2,3 ; Wang, Lingling 2; Li, Chenghua 1; Song, Linsheng 4
刊名	FISH & SHELLFISH IMMUNOLOGY
	2015-06-01
卷号	44 期号:2 页码:547-554
关键词	Crassostrea gigas Interferon Poly (I: C) Apoptosis Phagocytosis Anti-proliferation
英文摘要	Interferons (IFNs) belong to class II helical cytokines family with pleiotropic biological activities, which have been demonstrated to play crucial roles in innate and adaptive immunity in vertebrates. In the present study, a novel IFN-like protein (designed CgIFNLP) was identified from oyster Crassostrea gigas, which contained an interferon domain from 14 to 97 amino acids showing low sequence similarities with vertebrates IFNs, but shared a similar three-dimensional structure with class II helical cytokines. The mRNA transcripts of CgIFNLP was detected in all the tested tissues including gonad, adductor muscle, hemocytes, mantle, gills, and hepatopancreas, with the highest expression level in gills (39-fold, P < 0.05). Moreover, the expression level of CgIFNLP mRNA in hemocytes increased significantly at 12 h (8.35-fold, P < 0.01) and 24 h (4.95-fold, P < 0.01) after poly (I: C) stimulation. After the treatments by recombinant CgIFNLP protein (rCgIFNLP) at different concentrations, the apoptosis and phagocytosis rates of oyster hemocytes increased obviously. The proliferation rate of L929 did not change obviously after incubation with rCgIFNLP for 72 h, but the proliferation rate of A549 abated significantly at 36 h and 48 h after incubation with rCgIPNLP. The results collectively suggested that the IFN-like molecule existed in oyster and it tended to present conserved functions rather than conserved amino acid sequence in comparison with vertebrate IFNs. (c) 2015 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000354583800019
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/26966]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Zhejiang, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Dalian Ocean Univ, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ran,Liu, Rui,Wang, Weilin,et al. Identification and functional analysis of a novel IFN-like protein (CgIFNLP) in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2015,44(2):547-554.
APA	Zhang, Ran.,Liu, Rui.,Wang, Weilin.,Xin, Lusheng.,Wang, Lingling.,...&Song, Linsheng.(2015).Identification and functional analysis of a novel IFN-like protein (CgIFNLP) in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,44(2),547-554.
MLA	Zhang, Ran,et al."Identification and functional analysis of a novel IFN-like protein (CgIFNLP) in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 44.2(2015):547-554.