Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes | |
Ji, Xiaoyuan1; Su, Zhiguo1; Wang, Ping1,2,3; Ma, Guanghui1; Zhang, Songping1 | |
刊名 | ACS NANO |
2015-04-01 | |
卷号 | 9期号:4页码:4600-4610 |
关键词 | carbon dioxide bioconversion cofactor regeneration hollow nanofibers multienzyme system |
ISSN号 | 1936-0851 |
英文摘要 | Enzymatic conversion of carbon dioxide (CO2) to fuel or chemicals is appealing, but is limited by lack of efficient technology for regeneration and reuse of expensive cofactors. Here we show that cationic polyelectrolyte-doped hollow nanofibers, which can be fabricated via a facile coaxial electrospinning technology, provide an ideal scaffold for assembly of cofactor and multienzymes capable of synthesizing methanol from CO2 through a cascade multistep reaction involving cofactor regeneration. Cofactor and four enzymes including formate, formaldehyde, alcohol, and glutamate dehydrogenases were in situ coencapsulated inside the lumen of hollow nanofibers by involving them in the core-phase solution for coaxial electrospinning, in which cationic polyelectrolyte was predissolved. The polyelectrolyte penetrating across the shell of the hollow nanofibers enabled efficient tethering and retention of cofactor inside the lumen via ion-exchange interactions between oppositely charged polyelectrolytes and cofactor. With carbonic anhydrase assembled on the outer surface of the hollow nanofibers for accelerating hydration Of CO2, these five-enzymes-cofactor catalyst system exhibited high activity for methanol synthesis. Compared with methanol yield of only 36.17% using free enzymes and cofactor, the hollow nanofiber-supported system afforded a high value up to 103.2%, the highest reported value so far. It was believed that the linear polyelectrolytes acted as spacers to enhance the shuttling of cofactor between enzymes that were coencapsulated within near vicinity, thus improving the efficiency of the system. The immobilized system showed good stability in reusing. About 80% of its original productivity was retained after 10 reusing cycles, with a cofactor-based cumulative methanol yield reached 940.5%. |
WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
类目[WOS] | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
研究领域[WOS] | Chemistry ; Science & Technology - Other Topics ; Materials Science |
关键词[WOS] | SITU COFACTOR REGENERATION ; GLUCOSE-DEHYDROGENASE ; EFFICIENT CONVERSION ; MEMBRANE REACTOR ; ARTIFICIAL CELLS ; BIENZYME SYSTEM ; CO2 ; REDUCTION ; ANHYDRASE ; ENZYMES |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000353867000129 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/16770] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Minnesota, Dept Bioprod & Biosyst Engn, St Paul, MN 55108 USA 3.Univ Minnesota, Inst Biotechnol, St Paul, MN 55108 USA |
推荐引用方式 GB/T 7714 | Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,et al. Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes[J]. ACS NANO,2015,9(4):4600-4610. |
APA | Ji, Xiaoyuan,Su, Zhiguo,Wang, Ping,Ma, Guanghui,&Zhang, Songping.(2015).Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes.ACS NANO,9(4),4600-4610. |
MLA | Ji, Xiaoyuan,et al."Tethering of Nicotinamide Adenine Dinucleotide Inside Hollow Nanofibers for High-Yield Synthesis of Methanol from Carbon Dioxide Catalyzed by Coencapsulated Multienzymes".ACS NANO 9.4(2015):4600-4610. |
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