Grouper tsh beta Promoter-Driven Transgenic Zebrafish Marks Proximal Kidney Tubule Development

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	Grouper tsh beta Promoter-Driven Transgenic Zebrafish Marks Proximal Kidney Tubule Development
	Wang, Yang; Sun, Zhi-Hui; Zhou, Li; Li, Zhi; Gui, Jian-Fang
刊名	PLOS ONE
	2014-06-06
卷号	9 期号:6
关键词	DIFFERENTIAL EXPRESSION TRANSCRIPTION FACTOR THYROTROPIN RECEPTOR NEPHRON SEGMENTATION SEX REVERSAL CELL FATE PRONEPHROS GENE SPECIFICATION VERTEBRATE
ISSN号	1932-6203
英文摘要	Kidney tubule plays a critical role in recovering or secreting solutes, but the detailed morphogenesis remains unclear. Our previous studies have found that grouper tsh beta (gtsh beta) is also expressed in kidney, however, the distribution significance is still unknown. To understand the gtshb role and kidney tubule morphogenesis, here, we have generated a transgenic zebrafish line Tg(gtsh beta: GFP) with green fluorescent protein driven by the gtshb promoter. Similar to the endogenous tshb in zebrafish or in grouper, the gtsh beta promoter-driven GFP is expressed in pituitary and kidney, and the developing details of proximal kidney tubule are marked in the transgenic zebrafish line. The gfp initially transcribes at 16 hours post fertilization (hpf) above the dorsal mesentery, and partially co-localizes with pronephric tubular markers slc20a1a and cdh17. Significantly, the GFP specifically localizes in proximal pronephric segments during embryogenesis and resides at kidney duct epithelium in adult fish. To test whether the gtsh beta promoter-driven GFP may serve as a readout signal of the tubular development, we have treated the embryos with retinoic acid signaing (RA) reagents, in which exogenous RA addition results in a distal extension of the proximal segments, while RA inhibition induces a weakness and shortness of the proximal segments. Therefore, this transgenic line provides a useful tool for genetic or chemical analysis of kidney tubule.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	DIFFERENTIAL EXPRESSION ; TRANSCRIPTION FACTOR ; THYROTROPIN RECEPTOR ; NEPHRON SEGMENTATION ; SEX REVERSAL ; CELL FATE ; PRONEPHROS ; GENE ; SPECIFICATION ; VERTEBRATE
收录类别	SCI
语种	英语
WOS记录号	WOS:000341869000013
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20351]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yang,Sun, Zhi-Hui,Zhou, Li,et al. Grouper tsh beta Promoter-Driven Transgenic Zebrafish Marks Proximal Kidney Tubule Development[J]. PLOS ONE,2014,9(6).
APA	Wang, Yang,Sun, Zhi-Hui,Zhou, Li,Li, Zhi,&Gui, Jian-Fang.(2014).Grouper tsh beta Promoter-Driven Transgenic Zebrafish Marks Proximal Kidney Tubule Development.PLOS ONE,9(6).
MLA	Wang, Yang,et al."Grouper tsh beta Promoter-Driven Transgenic Zebrafish Marks Proximal Kidney Tubule Development".PLOS ONE 9.6(2014).