RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli

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	RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli
	Guo, YX ; Quiroga, C ; Chen, Q ; McAnulty, MJ ; Benedik, MJ ; Wood, TK ; Wang, XX
刊名	NUCLEIC ACIDS RESEARCH
	2014
卷号	42 期号:10 页码:6448-6462
ISSN号	0305-1048
通讯作者	twood@engr.psu.edu ; xxwang@scsio.ac.cn
中文摘要	For toxin/antitoxin (TA) systems, no toxin has been identified that functions by cleaving DNA. Here, we demonstrate that RalR and RalA of the cryptic prophage rac form a type I TA pair in which the antitoxin RNA is a trans-encoded small RNA with 16 nucleotides of complementarity to the toxin mRNA. We suggest the newly discovered antitoxin gene be named ralA for RalR antitoxin. Toxin RalR functions as a non-specific endonuclease that cleaves methylated and unmethylated DNA. The RNA chaperone Hfq is required for RalA antitoxin activity and appears to stabilize RalA. Also, RalR/RalA is beneficial to the Escherichia coli host for responding to the antibiotic fosfomycin. Hence, our results indicate that cryptic prophage genes can be functionally divergent from their active phage counterparts after integration into the host genome.
学科主题	Biochemistry & Molecular Biology
收录类别	SCI
资助信息	National Basic Research Program of China [2013CB955701]; National Natural Science Foundation of China [NFSC31270214, NFSC31290233]; National Institutes of Health [NIH R01 GM089999]; 1000-Youth Elite Program (the Recruitment Program of Global Experts in China)
原文出处	OXFORD UNIV PRESS
WOS记录号	WOS:000338768100034
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10481]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Guo, YX,Quiroga, C,Chen, Q,et al. RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli[J]. NUCLEIC ACIDS RESEARCH,2014,42(10):6448-6462.
APA	Guo, YX.,Quiroga, C.,Chen, Q.,McAnulty, MJ.,Benedik, MJ.,...&Wang, XX.(2014).RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli.NUCLEIC ACIDS RESEARCH,42(10),6448-6462.
MLA	Guo, YX,et al."RalR (a DNase) and RalA (a small RNA) form a type I toxin-antitoxin system in Escherichia coli".NUCLEIC ACIDS RESEARCH 42.10(2014):6448-6462.