Metabolic Engineering of Escherichia coli for the Production of Xylonate

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 材料生物技术研究中心

	Metabolic Engineering of Escherichia coli for the Production of Xylonate
	Cao, Yujin; Xian, Mo; Zou, Huibin; Zhang, Haibo
刊名	PLOS ONE
	2013-07-05
卷号	8 期号:7 页码:1-7
英文摘要	Xylonate is a valuable chemical for versatile applications. Although the chemical synthesis route and microbial conversion pathway were established decades ago, no commercial production of xylonate has been obtained so far. In this study, the industrially important microorganism Escherichia coli was engineered to produce xylonate from xylose. Through the coexpression of a xylose dehydrogenase (xdh) and a xylonolactonase (xylC) from Caulobacter crescentus, the recombinant strain could convert 1 g/L xylose to 0.84 g/L xylonate and 0.10 g/L xylonolactone after being induced for 12 h. Furthermore, the competitive pathway for xylose catabolism in E. coli was blocked by disrupting two genes (xylA and xylB) encoding xylose isomerase and xylulose kinase. Under fed-batch conditions, the finally engineered strain produced up to 27.3 g/L xylonate and 1.7 g/L xylonolactone from 30 g/L xylose, about 88% of the theoretical yield. These results suggest that the engineered E. coli strain has a promising perspective for large-scale production of xylonate.
学科主题	生物基化学品
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	D-XYLOSE METABOLISM ; SACCHAROMYCES-CEREVISIAE ; PSEUDOMONAS-FRAGI ; ACID PRODUCTION ; DEHYDROGENASE ; GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000321425300010
公开日期	2014-03-21
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1658]
专题	青岛生物能源与过程研究所_材料生物技术研究中心
作者单位	Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Cao, Yujin,Xian, Mo,Zou, Huibin,et al. Metabolic Engineering of Escherichia coli for the Production of Xylonate[J]. PLOS ONE,2013,8(7):1-7.
APA	Cao, Yujin,Xian, Mo,Zou, Huibin,&Zhang, Haibo.(2013).Metabolic Engineering of Escherichia coli for the Production of Xylonate.PLOS ONE,8(7),1-7.
MLA	Cao, Yujin,et al."Metabolic Engineering of Escherichia coli for the Production of Xylonate".PLOS ONE 8.7(2013):1-7.