Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica

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	Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica
	Zhang, Dong Ling 1,2,3; Guan, Rui Zhang 1,2; Huang, Wen Shu 2; Xiong, Jing 2
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-09-01
卷号	35 期号:3 页码:625-631
关键词	Antibacterial peptide Hemoglobin alpha chain Japanese eel Anguilla japonica Liver
ISSN号	1050-4648
通讯作者	Guan, RZ (reprint author), Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China.
中文摘要	We isolated and characterized a novel antibacterial peptide, AJHb alpha, derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica. It with concentration of 11.30 mu M exhibited stronger antibacterial activity against pathogenic bacterium 1 x 10(6) cell ml(-1) Edwardsiella tarda than other two bacteria. The extraction procedure for AJHb alpha included extraction with acetate acid, ultrafiltration, cation-exchange chromatography on HiTrap (TM) CM FF, reverse-phase liquid chromatography on Source 5R RPC and 08 RP-HPLC. MALDI-TOF MS suggested that the peptide had an observed molecular weight of 2388.05 Da. Its amino acid sequence determined by Edman degradation was similar to those of hemoglobin alpha chain in other fish by BLAST analysis. A complete N-terminal amino acid sequence of the AJHba was FAHWPDLGPGSPSVKKHGKVIM corresponding to the cDNA sequence by RACE amplification. Its synthetic peptide had strong antibacterial activities against ten Gram-positive or negative bacteria. To our knowledge, AJHba was the first identified fragment of hemoglobin alpha chain with strong antibacterial activity in fish. (C) 2012 Elsevier Ltd. All rights reserved.
英文摘要	We isolated and characterized a novel antibacterial peptide, AJHb alpha, derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica. It with concentration of 11.30 mu M exhibited stronger antibacterial activity against pathogenic bacterium 1 x 10(6) cell ml(-1) Edwardsiella tarda than other two bacteria. The extraction procedure for AJHb alpha included extraction with acetate acid, ultrafiltration, cation-exchange chromatography on HiTrap (TM) CM FF, reverse-phase liquid chromatography on Source 5R RPC and 08 RP-HPLC. MALDI-TOF MS suggested that the peptide had an observed molecular weight of 2388.05 Da. Its amino acid sequence determined by Edman degradation was similar to those of hemoglobin alpha chain in other fish by BLAST analysis. A complete N-terminal amino acid sequence of the AJHba was FAHWPDLGPGSPSVKKHGKVIM corresponding to the cDNA sequence by RACE amplification. Its synthetic peptide had strong antibacterial activities against ten Gram-positive or negative bacteria. To our knowledge, AJHba was the first identified fragment of hemoglobin alpha chain with strong antibacterial activity in fish. (C) 2012 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	RED-SEA BREAM ; ANTIMICROBIAL PEPTIDES ; ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; DEFENSE PEPTIDES ; EPIDERMAL MUCUS ; HISTONE H2A ; SKIN MUCUS ; CELLS ; FISH
收录类别	SCI
资助信息	Agricultural eel Public Welfare research project [nyhyzx07-043]; Department of Education of Fujian Province [JA09157]; Hall of Fujian Province Science and Technology platform construction project [2009N2003-1]; Fujian Province Science and Technology project [2010N0022]
语种	英语
WOS记录号	WOS:000324511700001
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19575]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2.Jimei Univ, Coll Fisheries, Xiamen 361021, Fujian Province, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Dong Ling,Guan, Rui Zhang,Huang, Wen Shu,et al. Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(3):625-631.
APA	Zhang, Dong Ling,Guan, Rui Zhang,Huang, Wen Shu,&Xiong, Jing.(2013).Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica.FISH & SHELLFISH IMMUNOLOGY,35(3),625-631.
MLA	Zhang, Dong Ling,et al."Isolation and characterization of a novel antibacterial peptide derived from hemoglobin alpha in the liver of Japanese eel, Anguilla japonica".FISH & SHELLFISH IMMUNOLOGY 35.3(2013):625-631.