Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array

doi:10.1016/j.aca.2024.342378

	Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array
	Zhang, Han 1,4; Mou, Junsong 1,4; Ding, Jiawang 1,2,3; Qin, Wei 1,2,3
刊名	ANALYTICA CHIMICA ACTA
	2024-04-08
卷号	1297 页码:8
关键词	Antibiotic screening Bacteria apoptosis Potentiometric detection Peptide
ISSN号	0003-2670
DOI	10.1016/j.aca.2024.342378
通讯作者	Ding, Jiawang(jwding@yic.ac.cn)
英文摘要	Phenotypic antimicrobial susceptibility testing enables reliable antibiotic screening but requires multiple strategies to identify each phenotypic change induced by different bactericidal mechanisms. Bacteria apoptosis with typical phenotypic features has never been explored for antibiotic screening. Herein, we developed an antibiotic screening method based on the measurement of antibiotic-induced phosphatidylserine (PS) exposure of apoptotic bacteria. Phosphatidylserine externalization of E. coli that can be widely used as an apoptosis marker for antibiotics with different antibacterial mechanisms was explored. A positively charged PS-binding peptide was immobilized on magnetic beads (MBs) to recognize and capture apoptotic E. coli with PS externalization. Apoptotic E. coli binding led to the charge or charge density change of MBs-peptide, resulting in a potential change on a magneto-controlled polymeric membrane potentiometric sensor. Based on the detection of apoptotic E. coli killed by antibiotics, antibiotic screening for different classes of antibiotics and silver nanoparticles was achieved within 1.5 h using a potentiometric sensor array. This approach enables sensitive, general, and timesaving antibiotic screening, and may open up a new path for antibiotic susceptibility testing.
WOS关键词	ANTIBACTERIAL ACTIVITY ; CELL-DEATH ; SILVER ; RESISTANCE ; NANOPARTICLES ; MECHANISMS ; BACTERIA ; DOTS
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:001193783100001
资助机构	National Natural Science Foundation of China ; Taishan Scholar Program of Shandong Province ; Special Fund for the Scholar Program of Yantai and Shandong Province ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34937]
专题	中国科学院烟台海岸带研究所
通讯作者	Ding, Jiawang
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Qingdao 266237, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array[J]. ANALYTICA CHIMICA ACTA,2024,1297:8.
APA	Zhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2024).Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array.ANALYTICA CHIMICA ACTA,1297,8.
MLA	Zhang, Han,et al."Rapid antibiotic screening based on E. coli apoptosis using a potentiometric sensor array".ANALYTICA CHIMICA ACTA 1297(2024):8.