Joint-detection of Salmonella typhimurium and Escherichia coli O157:H7 by an immersible amplification dip-stick immunoassay

	Joint-detection of Salmonella typhimurium and Escherichia coli O157:H7 by an immersible amplification dip-stick immunoassay
	Chen, Yaqian; Ma, Jiaqi; Yin, Xuechi; Deng, Ziai; Liu, Xiaojing; Yang, Di; Zhao, Lei; Sun, Jing; Wang, Jianlong; Zhang, Daohong
刊名	BIOSENSORS & BIOELECTRONICS
	2023
卷号	224
英文摘要	To explore the superiority of multifunctional nanocomposites and realize the joint-detection of foodborne pathogens, an immersible amplification dip-stick immunoassay (DSIA) was exploited for the sensitive detection of Salmonella typhimurium (S. typhi) and Escherichia coli O157:H7 (E. coli O157:H7). Saving for the basic colorimetric performance, the reporter molecule of CoFe2O4 (CFO) possesses multivalent elements (Co2+/3+, Fe2+/3+) as well as multifunction of superior catalase-like activity and magnetic properties. By dint of the catalytic activity of CFO, a directly immersible amplification can be simply achieved to endure the DSIA with an intensive signal and a dual-visible mode for the determination of S. typhi and E. coli O157:H7. In virtue of the magnetic separation and enrichment capability of the CFO, the DSIA can perform a matrix-interference-free detection and obtain a dynamic detection range of 102-108 CFU/mL and a low assay limit of 102 CFU/mL. Moreover, the DSIA has reasonable recovery rates for contamination monitoring of two target bacteria in milk and beef samples. Our research provides a persuasive supplement for the application of multifunctional nanocomposites in the ongoing dip-stick immunoassay and an alternative strategy for the efficient detection of foodborne pathogens.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61711]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chen, Yaqian,Ma, Jiaqi,Yin, Xuechi,et al. Joint-detection of Salmonella typhimurium and Escherichia coli O157:H7 by an immersible amplification dip-stick immunoassay[J]. BIOSENSORS & BIOELECTRONICS,2023,224.
APA	Chen, Yaqian.,Ma, Jiaqi.,Yin, Xuechi.,Deng, Ziai.,Liu, Xiaojing.,...&Zhang, Daohong.(2023).Joint-detection of Salmonella typhimurium and Escherichia coli O157:H7 by an immersible amplification dip-stick immunoassay.BIOSENSORS & BIOELECTRONICS,224.
MLA	Chen, Yaqian,et al."Joint-detection of Salmonella typhimurium and Escherichia coli O157:H7 by an immersible amplification dip-stick immunoassay".BIOSENSORS & BIOELECTRONICS 224(2023).