Induce male sterility by CRISPR/Cas9-mediated mitochondrial genome editing in tobacco

	Induce male sterility by CRISPR/Cas9-mediated mitochondrial genome editing in tobacco
	Chang, Yanzi; Liu, Baolong; Jiang, Yanyan; Cao, Dong; Liu, Yongju; Li, Yun
刊名	FUNCTIONAL & INTEGRATIVE GENOMICS
	2023
卷号	23 期号:3
英文摘要	Genome editing has become more and more popular in animal and plant systems following the emergence of CRISPR/Cas9 technology. However, target sequence modification by CRISPR/Cas9 has not been reported in the plant mitochondrial genome, mtDNA. In plants, a type of male sterility known as cytoplasmic male sterility (CMS) has been associated with certain mitochondrial genes, but few genes have been confirmed by direct mitochondrial gene-targeted modifications. Here, the CMS-associated gene (mtatp9) in tobacco was cleaved using mitoCRISPR/Cas9 with a mitochondrial localization signal. The male-sterile mutant, with aborted stamens, exhibited only 70% of the mtDNA copy number of the wild type and exhibited an altered percentage of heteroplasmic mtatp9 alleles; otherwise, the seed setting rate of the mutant flowers was zero. Transcriptomic analyses showed that glycolysis, tricarboxylic acid cycle metabolism and the oxidative phosphorylation pathway, which are all related to aerobic respiration, were inhibited in stamens of the male-sterile gene-edited mutant. In addition, overexpression of the synonymous mutations dsmtatp9 could restore fertility to the male-sterile mutant. Our results strongly suggest that mutation of mtatp9 causes CMS and that mitoCRISPR/Cas9 can be used to modify the mitochondrial genome of plants.
内容类型	期刊论文
源URL	[http://210.75.249.4/handle/363003/61581]
专题	西北高原生物研究所_中国科学院西北高原生物研究所
推荐引用方式 GB/T 7714	Chang, Yanzi,Liu, Baolong,Jiang, Yanyan,et al. Induce male sterility by CRISPR/Cas9-mediated mitochondrial genome editing in tobacco[J]. FUNCTIONAL & INTEGRATIVE GENOMICS,2023,23(3).
APA	Chang, Yanzi,Liu, Baolong,Jiang, Yanyan,Cao, Dong,Liu, Yongju,&Li, Yun.(2023).Induce male sterility by CRISPR/Cas9-mediated mitochondrial genome editing in tobacco.FUNCTIONAL & INTEGRATIVE GENOMICS,23(3).
MLA	Chang, Yanzi,et al."Induce male sterility by CRISPR/Cas9-mediated mitochondrial genome editing in tobacco".FUNCTIONAL & INTEGRATIVE GENOMICS 23.3(2023).