Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

doi:10.1007/s00343-022-1358-0

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus
	Jiao, Shuang 3; Tan, Xungang 1,2; You, Feng 1,2; Zhang, Shujing 3; Pang, Qiuxiang 3
刊名	JOURNAL OF OCEANOLOGY AND LIMNOLOGY
	2022-09-20
页码	10
关键词	aquaculture flatfish igf2 proliferation
ISSN号	2096-5508
DOI	10.1007/s00343-022-1358-0
通讯作者	Jiao, Shuang(jiaoshuang@sdut.edu.cn) ; Tan, Xungang(tanx@qdio.ac.cn)
英文摘要	Insulin-like growth factors (IGFs) are key regulators of development and growth. Here, we characterized the igf2 gene from olive flounder (Paralichthys olivaceus) and determined its temporal and spatial expression. We set up an in-vitro protein expression system in eukaryotic human embryonic kidney (HEK293T) cells and explored its effects on cell proliferation. The flounder igf2 cDNA contained a 648-bp open reading frame (ORF) encoding a protein of 215 amino acids (aa), which spanned the complete signal peptide (47 aa), mature peptide (70 aa), and E domain (98 aa). In adult flounder, igf2 mRNA was detected in all selected tissues. In early development, igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage embryos. In-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region, floor plate, hypochord, otic vesicle, and pectoral fin during embryogenesis. Western blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T cells. In addition, the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma (HeLa) and HEK293T cells. These results provide new information about the structural and functional conservation, expression patterns, and biological activity of the igf2 in teleosts.
资助项目	National Natural Science Foundation of China[31672636] ; Key Research and Development Program of Shandong Province, China[2019GHY112007]
WOS研究方向	Marine & Freshwater Biology ; Oceanography
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000855632500003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/180804]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Jiao, Shuang; Tan, Xungang
作者单位	1.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Shandong Univ Technol, Sch Life Sci & Med, Antiaging & Regenerat Med Res Inst, Zibo 255000, Peoples R China
推荐引用方式 GB/T 7714	Jiao, Shuang,Tan, Xungang,You, Feng,et al. Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2022:10.
APA	Jiao, Shuang,Tan, Xungang,You, Feng,Zhang, Shujing,&Pang, Qiuxiang.(2022).Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10.
MLA	Jiao, Shuang,et al."Molecular identification, expression pattern, and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2022):10.