Comprehensive analysis of the expression, prognosis, and immune infiltrates for CHDs in human lung cancer

doi:10.1007/s12672-022-00489-y

	Comprehensive analysis of the expression, prognosis, and immune infiltrates for CHDs in human lung cancer
	Lv, Yang 1,2,3; Lin, Wenchu 1,3
刊名	DISCOVER ONCOLOGY
	2022-04-25
卷号	13
关键词	Lung cancer CHD family Prognosis Bioinformatics analysis Immune infiltrates
ISSN号	1868-8497
DOI	10.1007/s12672-022-00489-y
通讯作者	Lin, Wenchu(wenchu@hmfl.ac.cn)
英文摘要	Background The chromodomain helicase DNA-binding (CHD) family, a group of genes that regulate nucleosome spacing and access to transcription factors, contributes to tumorigenesis in various cancers. However, the roles of CHD family members in lung cancer remain poorly understood. Methods We investigated the transcriptional, survival, and immune data of CHDs in patients with lung cancer from the Oncomine, UALCAN, GEPIA, Kaplan-Meier Plotter, TCGA, TIMER, cBioPortal, and CR2Cancer databases. Then, perform functional enrichment analysis of CHDs was performed using the Metascape. Finally, the expression of CHD7, CHD8 and DNA damage response genes were evaluated by quantitative real-time PCR and western blot.The effects of CHD7 or CHD8 knockdown on A549 and PC9 cells were measured in vitro by flow cytometry, cell viability and colony formation assays. Results We found that except for CHD5, nearly all members of CHDs in lung cancer showed altered expression compared with adjacent normal tissues. Moreover, the abnormal expression levels of CHDs were related to the clinical outcome of patients with lung adenocarcinoma and, to a lesser extent, patients with lung squamous cell carcinoma, which were significantly associated with the immune infiltrating levels of immune cells. Furthermore, the functions of CHDs and their neighboring genes are mainly related to DNA repair, the cell cycle, and organelle organization. Finally, cellular experiments conducted in vitro confirmed that CHD7/8 played indispensable roles in DNA damage signaling and cell cycle progression in lung adenocarcinoma cells. Conclusion This study implied that CHD family members, especially in subclass III, are potential targets of precision therapy and new biomarkers for patients with lung cancer.
资助项目	National Natural Science Foundation of China[81972191] ; National Natural Science Foundation of China[81672647] ; Science and Technology Major Project of Anhui Province[18030801140] ; 100-Talent Program of Chinese Academy of Sciences ; High Magnetic Field Laboratory of Anhui Province
WOS关键词	DNA-BINDING PROTEIN ; TUMOR-SUPPRESSOR ; GENE-EXPRESSION ; CHROMATIN REGULATORS ; WEB SERVER ; CHROMODOMAIN ; DATABASE ; FAMILY
WOS研究方向	Oncology ; Endocrinology & Metabolism
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000787297900001
资助机构	National Natural Science Foundation of China ; Science and Technology Major Project of Anhui Province ; 100-Talent Program of Chinese Academy of Sciences ; High Magnetic Field Laboratory of Anhui Province
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131484]
专题	中国科学院合肥物质科学研究院
通讯作者	Lin, Wenchu
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, High Field Magnet Lab, Hefei 230031, Anhui, Peoples R China 2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China 3.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei 230031, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Lv, Yang,Lin, Wenchu. Comprehensive analysis of the expression, prognosis, and immune infiltrates for CHDs in human lung cancer[J]. DISCOVER ONCOLOGY,2022,13.
APA	Lv, Yang,&Lin, Wenchu.(2022).Comprehensive analysis of the expression, prognosis, and immune infiltrates for CHDs in human lung cancer.DISCOVER ONCOLOGY,13.
MLA	Lv, Yang,et al."Comprehensive analysis of the expression, prognosis, and immune infiltrates for CHDs in human lung cancer".DISCOVER ONCOLOGY 13(2022).