Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy br

doi:10.1021/acs.analchem.2c00773

	Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy br
	Sun, Yue 1,2; Zhang, Zhicheng 1,2; Bing, Tao 3,4; Liu, Jing 2,3; Li, Weiwei 4,5; Liu, Xiangjun 2,3; Zhang, Nan 2,3; Shu, Yang 1; Wang, Jianhua 1; Shangguan, Dihua 2,3,5
刊名	ANALYTICAL CHEMISTRY
	2022-04-19
卷号	94
ISSN号	0003-2700
DOI	10.1021/acs.analchem.2c00773
通讯作者	Bing, Tao(bingtao@iccas.ac.cn) ; Shu, Yang(shuyang@mail.neu.edu.cn) ; Shangguan, Dihua(sgdh@iccas.ac.cn)
英文摘要	Expansion microscopy (ExM) is a newly developed technology inrecent years that enables nanoscale imaging under conventional microscopes. Herein,we report an aptamer-based ExM imaging strategy. A nucleus-targeting aptamer Ch4-1 was chemically labeled with a dye and an acrydite at each end to perform thefunctions of molecular recognition,fluorescence reporting, and gel anchoring. Afterbinding cell nucleus, the dual labeled aptamer Ac-Ch4-1-FAM directly participated ingelation and anchored in polyacrylamide gel. After expanding the gel, high-resolutionimaging was achieved by confocal microscopy. Multicolor ExM imaging was alsorealized by combining Ac-Ch4-1-FAM, antibodies andfluorescent dyes. This aptamer-based ExM could clearly image the chromatinmorphology at different mitotic stages. The expansion process is simple and the aptamer labeling is easy. The aptamer-based ExMholds great promise in super-resolution imaging of cells and tissues.
资助项目	National Natural Science Foundation of China[21874140] ; National Natural Science Foundation of China[21635008] ; National Natural Science Foundation of China[22174149] ; National Natural Science Foundation of China[21877115]
WOS关键词	NANOSCALE ; REVEALS ; PROBES
WOS研究方向	Chemistry
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000792814500040
资助机构	National Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/131095]
专题	中国科学院合肥物质科学研究院
通讯作者	Bing, Tao; Shu, Yang; Shangguan, Dihua
作者单位	1.Northeastern Univ, Dept Chem, Coll Sci, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst,CAS Res Educ C, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Canc Hosp, Univ Chinese Acad Sci, Zhejiang Canc Hosp,Inst Basic Med & Canc IBMC, Hangzhou 310022, Zhejiang, Peoples R China 5.Univ Chinese Acad Sci, Sch Mol Med, Hangzhou Inst Adv Study, Hangzhou 310013, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yue,Zhang, Zhicheng,Bing, Tao,et al. Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy br[J]. ANALYTICAL CHEMISTRY,2022,94.
APA	Sun, Yue.,Zhang, Zhicheng.,Bing, Tao.,Liu, Jing.,Li, Weiwei.,...&Shangguan, Dihua.(2022).Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy br.ANALYTICAL CHEMISTRY,94.
MLA	Sun, Yue,et al."Aptamer-Based Cell Nucleus Imaging via Expansion Microscopy br".ANALYTICAL CHEMISTRY 94(2022).