High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli

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	High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli
	Wang, Dan 1,2; Li, Qiang 1,2; Mao, Yu 3; Xing, Jianmin 1; Su, Zhiguo 1
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2010-08-01
卷号	87 期号:6 页码:2025-2035
关键词	Glucose phosphotransferase (ptsG) Escherichia coli Succinic acid Lactose induction Phosphoenolpyruvate carboxylase (ppc)
ISSN号	0175-7598
通讯作者	Xing, JM
英文摘要	Escherichia coli strains with foreign genes under the isopropyl-beta-d-thiogalactopyranoside-inducible promoters such as lac, tac, and trc were engineered and considered as the promising succinic acid-producing bacteria in many reports. The promoters mentioned above could also be induced by lactose, which had not been attempted for succinic acid production before. Here, the efficient utilization of lactose as inducer was demonstrated in cultures of the ptsG, ldhA, and pflB mutant strain DC1515 with ppc overexpression. A fermentative process for succinic acid production at high level by this strain was developed. In flask anaerobic culture, 14.86 g l(-1) succinic acid was produced from 15 g l(-1) glucose with a yield of 1.51 mol mol(-1) glucose. In two-stage culture carried out in a 3-l bioreactor, the overall yield and concentration of succinic acid reached to 1.67 mol mol(-1) glucose and 99.7 g l(-1), respectively, with a productivity of 1.7 g l(-1) h(-1) in the anaerobic stage. The efficient utilization of lactose as inducer made recombinant E. coli a more capable strain for succinic acid production at large scale.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	RECOMBINANT PROTEIN-PRODUCTION ; FED-BATCH CULTURE ; PYRUVATE-CARBOXYLASE ; LAC PROMOTER ; STRAIN ; OVEREXPRESSION ; GENE ; FERMENTATION ; METABOLISM ; GLUCOSE
收录类别	SCI
语种	英语
WOS记录号	WOS:000280373100005
公开日期	2013-11-15
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/5835]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.China Univ Min & Technol Beijing, Sch Chem & Environm Engn, Dept Bioengn, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Wang, Dan,Li, Qiang,Mao, Yu,et al. High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2010,87(6):2025-2035.
APA	Wang, Dan,Li, Qiang,Mao, Yu,Xing, Jianmin,&Su, Zhiguo.(2010).High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,87(6),2025-2035.
MLA	Wang, Dan,et al."High-level succinic acid production and yield by lactose-induced expression of phosphoenolpyruvate carboxylase in ptsG mutant Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 87.6(2010):2025-2035.