iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid

doi:10.3389/fmars.2021.792050

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	iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid
	Wang, Xin 2,3,4; Wang, Dan 2,3,4; Zhang, Tianyu 2,3,4; Zhang, Qianqian 1,3,4; Zhao, Jianmin 1,3,4
刊名	FRONTIERS IN MARINE SCIENCE
	2021-12-08
卷号	8 页码:12
关键词	okadaic acid Chlamys farreri oxidative stress detoxification genes iTRAQ
DOI	10.3389/fmars.2021.792050
通讯作者	Zhang, Qianqian(qqzhang@yic.ac.cn) ; Zhao, Jianmin(jmzhao@yic.ac.cn)
英文摘要	Okadaic acid (OA), produced by dinoflagellates during harmful algal blooms, is a principal diarrhetic shellfish poisoning toxin. This toxin poses a potential threat to bivalves with economic values. To better understand the toxicity mechanism of OA to bivalves, in this study, oxidative stress biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione S-transferase, GST; malondialdehyde, MDA) and the expression of detoxification genes (heat shock protein 70, HSP70; heat shock protein 90, HSP90; cytochrome P450, CYP450) were assessed in the gills of scallops Chlamys farreri after 24 h, 48 h and 96 h exposure to OA. In addition, the digestive glands of scallops exposed to OA for 96 h were dissected for an iTRAQ based quantitative proteomic analysis. The results of OA exposure experiments showed that OA induces oxidative stress and significant enhancement of the expression of detoxification genes in scallops. The proteomics analysis revealed that 159 proteins altered remarkably in OA-treated scallops, and these proteins were involved in phagosomes, regulation of actin cytoskeleton, adherens junction, tight junction, and focal adhesion. Amino acid biosynthesis, carbon metabolism, pentose phosphate pathway, fructose and mannose metabolism in the digestive glands were also significantly impacted. Our data shed new insights on the molecular responses and toxicity mechanisms of C. farreri to OA.
WOS关键词	MYTILUS-GALLOPROVINCIALIS ; OXIDATIVE STRESS ; GENE-EXPRESSION ; ALGAL BLOOMS ; SUPEROXIDE-DISMUTASE ; ANTIOXIDANT ENZYMES ; PROROCENTRUM-LIMA ; MARINE TOXINS ; UNIO-TUMIDUS ; F-ACTIN
WOS研究方向	Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种	英语
WOS记录号	WOS:000732816700001
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31531]
专题	中国科学院牟平海岸带环境综合试验站
通讯作者	Zhang, Qianqian; Zhao, Jianmin
作者单位	1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xin,Wang, Dan,Zhang, Tianyu,et al. iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid[J]. FRONTIERS IN MARINE SCIENCE,2021,8:12.
APA	Wang, Xin,Wang, Dan,Zhang, Tianyu,Zhang, Qianqian,&Zhao, Jianmin.(2021).iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid.FRONTIERS IN MARINE SCIENCE,8,12.
MLA	Wang, Xin,et al."iTRAQ-Based Quantitative Proteomic Analysis Reveals Toxicity Mechanisms in Chlamys farreri Exposed to Okadaic Acid".FRONTIERS IN MARINE SCIENCE 8(2021):12.