Characterization of the differentiated reduction of selenite and tellurite by a halotolerant bacterium: Process and mechanism

doi:10.1016/j.jwpe.2022.102809

	Characterization of the differentiated reduction of selenite and tellurite by a halotolerant bacterium: Process and mechanism
	Cheng, Manman 1,4; Sun, Yanyu 4; Sui, Xiaori 4; Zhang, Haikun 2,3,4
刊名	JOURNAL OF WATER PROCESS ENGINEERING
	2022-06-01
卷号	47 页码:10
关键词	Se(IV) Te(IV) Reduction Marine bacterium Proteomics
ISSN号	2214-7144
DOI	10.1016/j.jwpe.2022.102809
通讯作者	Zhang, Haikun(hkzhang@yic.ac.cn)
英文摘要	Se(IV)-and Te(IV)-containing wastewater usually contains salt, which reduces the activity of conventional mi-croorganisms and may affect their microbial treatment. So far, salt-tolerant Se(IV)-and Te(IV)-reducing bacteria are very limited. Moreover, the detailed molecular response of microbial reduction of Se(IV)/Te(IV) under saline conditions has yet to be reported. In this study, the Se(IV) and Te(IV) reductive processes and mechanisms were investigated with the employment of a marine bacterium Shewanella sp. CNZ-1 (CNZ-1) using process and kinetic analyses, enzymology and RT-qPCR analyses, and differential proteomics approaches. Our results showed that CNZ-1 can effectively reduce Se(IV) and Te(IV) to Se0 and Te0 with the max k (R2) values of 0.0412 h-1 (0.86) and 0.0292 h(-1) (0.91) under 2% NaCl conditions, respectively. The mechanism study showed that CNZ-1 mediated Se(IV) reduction was an enzyme-based reductive reaction, whereas CNZ-1 mediated Te(IV) reduc-tion was more like a resistance-related detoxification process (including both bioadsorption and bioreduction). The bioreduction of Se(IV) was mainly depended on functional membrane proteins (> 4-fold; e.g., nitrite reductase, polysulfide reductase and fumarate reductase), while proteins related to efflux system and stress resistance system (> 2-fold; e.g., Type II secretory pathway and phage shock proteins) were essential for the Te (IV) bioreduction process. Overall, our findings expanded the understanding the fate of Se(IV) and Te(IV) in the saline environment and provided guidance for Se(IV) or Te(IV) pollution control.
WOS关键词	AQUEOUS-SOLUTION ; SHEWANELLA ; NANOPARTICLES ; BIOSYNTHESIS ; RECOVERY ; SELENATE ; REMOVAL ; SE ; PRECIPITATION ; NANOSPHERES
WOS研究方向	Engineering ; Water Resources
语种	英语
WOS记录号	WOS:000797861100011
资助机构	National Natural Science Foundation of China ; Youth Innovation Promotion Association CAS ; CSC Scholarship
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/31152]
专题	中国科学院烟台海岸带研究所
通讯作者	Zhang, Haikun
作者单位	1.Yantai Univ, Coll life Sci, Yantai 264000, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266000, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 4.Chinese Acad Sci, Yantai Inst Costal Zone Res, Yantai 264000, Peoples R China
推荐引用方式 GB/T 7714	Cheng, Manman,Sun, Yanyu,Sui, Xiaori,et al. Characterization of the differentiated reduction of selenite and tellurite by a halotolerant bacterium: Process and mechanism[J]. JOURNAL OF WATER PROCESS ENGINEERING,2022,47:10.
APA	Cheng, Manman,Sun, Yanyu,Sui, Xiaori,&Zhang, Haikun.(2022).Characterization of the differentiated reduction of selenite and tellurite by a halotolerant bacterium: Process and mechanism.JOURNAL OF WATER PROCESS ENGINEERING,47,10.
MLA	Cheng, Manman,et al."Characterization of the differentiated reduction of selenite and tellurite by a halotolerant bacterium: Process and mechanism".JOURNAL OF WATER PROCESS ENGINEERING 47(2022):10.