<p>Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp;</p>

doi:10.1016/j.algal.2022.102714

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp;
	Zheng, Zhenbing 1; He, Bangxiang 1,3; Guo, Meng Lin 1; Xie, Xiujun 1; Huan, Li 1; Zhang, Baoyu 1; Shao, Zhizhuo 1; Wang, Guangce 1,2
刊名	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
	2022-05-01
卷号	64 页码:10
关键词	One helix proteins Overexpression NPQ Neopyropia yezoensis Transgene
ISSN号	2211-9264
DOI	10.1016/j.algal.2022.102714
通讯作者	Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要	The red macroalga Neopyropia yezoensis is a model organism, and an economically important seaweed widely cultured in eastern Asian countries. Transgenic research on N. yezoensis is difficult and limited to reporter genes and selection markers due to the specificities of this species. In this study, plasmids were constructed to over express six one helix protein (OHP) coding genes in N. yezoensis, and four transgenic lines with high expression levels of the target genes were obtained. The photosynthetic parameters were determined and it was found that the overexpression of OHPs altered electron transport in photosystem I and photosystem II, which was indicated by the increased 1-qL values and decreased P700(+) reduction rates. The results also showed that the non photochemical quenching (NPQ) kinetics in N. yezoensis could not be stimulated by high light (HL) treatment. Two of the four transgenic lines showed stimulated NPQ, and they did not exhibit an obvious increased ability to resist to HL, which was shown by the decreased Fv/Fm values. The increased H2O2 content and decreased peroxidase (POD) activity were considered the possible results of the overexpressed OHPs, and they were responsible for the enhanced NPQ and HL sensitivity in those two transgenic lines.& nbsp;& nbsp;
资助项目	National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41976097]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000794241000013
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/179266]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Wang, Guangce
作者单位	1.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Anhui Polytech Univ, Coll Biol & Food Engn, Wuhu, Anhui, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Zhenbing,He, Bangxiang,Guo, Meng Lin,et al. Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp; [J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,64:10.
APA	Zheng, Zhenbing.,He, Bangxiang.,Guo, Meng Lin.,Xie, Xiujun.,Huan, Li.,...&Wang, Guangce.(2022). Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp; .ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,64,10.
MLA	Zheng, Zhenbing,et al." Overexpression of OHPs in Neopyropia yezoensis (Rhodophyta) reveals their possible physiological roles & nbsp; ".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 64(2022):10.