Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae

doi:10.3389/fmicb.2022.851182

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	Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae
	Li, Jiang 1; Gu, Xiaoqian 2; Zhang, Qian 1; Fu, Liping 1; Tan, Jiaojiao 2; Zhao, Luying 1
刊名	FRONTIERS IN MICROBIOLOGY
	2022-03-31
卷号	13 页码:11
关键词	carrageenase Antarctic macroalgae associated bacteria metagenome site-directed mutagenesis
DOI	10.3389/fmicb.2022.851182
通讯作者	Li, Jiang(lijiang@fio.org.cn)
英文摘要	A carrageenase gene, car1383, was obtained from the metagenome of Antarctic macroalgae-associated bacteria. The amino acid sequence of its product showed up to 33% similarity with other carrageenases and contained a GH16-family motif. The recombinant Car1383 was heterologously expressed in Eschericia coli and exhibited maximal activity at 50 degrees C and pH 6.0, with a K-m of 6.51 mg/ml and a V-max of 55.77 U/mg. Its activity was enhanced by some cations (Na+, K+, and Fe2+), but inhibited or inactivated by others (Sr2+, Ca2+, Ni2+, Ba2+, Mn2+, Cu2+, Fe3+, and Mg2+). Car1383 degraded carrageenan into neocarrabiose and neocarratetraose. Site-directed mutagenesis indicated that putative active sites, E-190 and E-195, conserved sites, W-183 and G(255), play important roles in Car1383 activity. This study provides a new candidate for the industrial preparation of bioactive algal oligosaccharides.
资助项目	Impact and Response of Antarctic Seas to Climate Change[RFSOCC2020-2022]
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000789133600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178876]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Jiang
作者单位	1.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Jiang,Gu, Xiaoqian,Zhang, Qian,et al. Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae[J]. FRONTIERS IN MICROBIOLOGY,2022,13:11.
APA	Li, Jiang,Gu, Xiaoqian,Zhang, Qian,Fu, Liping,Tan, Jiaojiao,&Zhao, Luying.(2022).Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae.FRONTIERS IN MICROBIOLOGY,13,11.
MLA	Li, Jiang,et al."Biochemical Characterization of a Carrageenase, Car1383, Derived From Associated Bacteria of Antarctic Macroalgae".FRONTIERS IN MICROBIOLOGY 13(2022):11.