Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)

doi:10.3389/fcimb.2022.875409

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)
	Li, Qian 2; Chi, Heng 1,2; Shi, Xueyan 2; Gan, Qiujie 2; Dalmo, Roy Ambli 3; Sun, Yuan-yuan 4; Tang, Xiaoqian 1,2; Xing, Jing 1,2; Sheng, Xiuzhen 1,2; Zhan, Wenbin 1,2
刊名	FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
	2022-03-30
卷号	12 页码:11
关键词	extracellular traps (ETs) antigen adjuvant vaccination fish
ISSN号	2235-2988
DOI	10.3389/fcimb.2022.875409
通讯作者	Chi, Heng(chiheng_2001@163.com)
英文摘要	Adjuvants are used to increase the strength, quality, and duration of the immune response of vaccines. Neutrophils are the first immune cells that arrive at the injection site and can release DNA fibers together with granular proteins, so-called neutrophil extracellular traps (NETs), to entrap microbes in a sticky matrix of extracellular chromatin and microbicidal agents. Similar extracellular structures were also released by macrophages, mast cells, and eosinophils and are now generalized as "ETs." Here we demonstrated that Alum adjuvant stimulation led to peritoneal cells swarming and ET release in vitro. Moreover, compared to antigen stimulation alone, ET release was significantly increased after stimulation with antigen-mixed adjuvants and in a time- and dose-dependent manner. In vivo, we were able to monitor and quantify the continuous changes of the ET release in the same fish by using the small animal in vivo imaging instrument at different times during the early stages after intraperitoneal immunization. The results showed that the fluorescence signal of ETs in the peritoneum increased from 0 to 12 h after injection and then gradually decreased. The fluorescence signals came from extracellular DNA fibers, which are sensitive to DNase I and confirmed by microscopy of peritoneal fluid ex vivo. In summary, this study introduced a new method for detecting ETs in the peritoneum of fish in vivo and indicated that ET formation is involved in the immune response at the early stage after intraperitoneal immunization to vaccines.
资助项目	National Natural Science Foundation of China[31872594] ; National Natural Science Foundation of China[31730101] ; National Key Research and Development Program of China[2019YFD0900101] ; National Key Research and Development Program of China[2019YFD0900102]
WOS研究方向	Immunology ; Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000784019200001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178777]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Chi, Heng
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 2.Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao, Peoples R China 3.Univ Tromso, Arctic Univ Norway, Norwegian Coll Fishery Sci, Fac Biosci Fisheries & Econ, Tromso, Norway 4.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Qian,Chi, Heng,Shi, Xueyan,et al. Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)[J]. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2022,12:11.
APA	Li, Qian.,Chi, Heng.,Shi, Xueyan.,Gan, Qiujie.,Dalmo, Roy Ambli.,...&Zhan, Wenbin.(2022).Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus).FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,12,11.
MLA	Li, Qian,et al."Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)".FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 12(2022):11.