Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor

doi:10.1111/cpr.12858

CORC > 长春光学精密机械与物理研究所 > 中国科学院长春光学精密机械与物理研究所

	Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor
	Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang
刊名	Cell Proliferation
	2020
卷号	53 期号:8 页码:12
ISSN号	0960-7722
DOI	10.1111/cpr.12858
英文摘要	Objectives Traditional cancer therapy and regular immunotherapy are ineffective for treating triple-negative breast cancer (TNBC) patients. Recently, chimeric antigen receptor-engineered natural killer cells (CAR NK) have been applied to target several hormone receptors on different cancer cells to improve the efficacy of immunotherapy. Furthermore, epidermal growth factor receptor (EGFR) is a potential therapeutic target for TNBC. Here, we demonstrated that EGFR-specific CAR NK cells (EGFR-CAR NK cells) could be potentially used to treat patients with TNBC exhibiting enhanced EGFR expression. Materials and methods We investigated the cytotoxic effects of EGFR-CAR NK cells against TNBC cells in vitro and in vivo. The two types of EGFR-CAR NK cells were generated by transducing lentiviral vectors containing DNA sequences encoding the single-chain variable fragment (scFv) regions of the two anti-EGFR antibodies. The cytotoxic and anti-tumor effects of the two cell types were examined by performing cytokine release and cytotoxicity assays in vitro, and tumor growth assays in breast cancer cell line-derived xenograft (CLDX) and patient-derived xenograft (PDX) mouse models. Results Both EGFR-CAR NK cell types were activated by TNBC cells exhibiting upregulated EGFR expression and specifically triggered the lysis of the TNBC cells in vitro. Furthermore, the two EGFR-CAR NK cell types inhibited CLDX and PDX tumors in mice. Conclusions This study suggested that treatment with EGFR-CAR NK cells could be a promising strategy for TNBC patients.
URL标识	查看原文
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/64945]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang. Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor[J]. Cell Proliferation,2020,53(8):12.
APA	Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang.(2020).Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor.Cell Proliferation,53(8),12.
MLA	Y. Liu,Y. H. Zhou,K. H. Huang,X. J. Fang,Y. Li,F. F. Wang,L. An,Q. F. Chen,Y. C. Zhang,A. H. Shi,S. Yu and J. Z. Zhang."Targeting epidermal growth factor-overexpressing triple-negative breast cancer by natural killer cells expressing a specific chimeric antigen receptor".Cell Proliferation 53.8(2020):12.