A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor

doi:10.3390/mi10100685

CORC > 长春光学精密机械与物理研究所 > 中国科学院长春光学精密机械与物理研究所

	A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor
	K.N.Wang; D.Wu; W.M.Wu
刊名	Micromachines
	2019
卷号	10 期号:10 页码:12
关键词	Microfluidics,viscosity,continuous flow,real-time PCR,driven,behavior,Science & Technology - Other Topics,Instruments & Instrumentation
DOI	10.3390/mi10100685
英文摘要	A self-activated micropump which is capable of stable velocity transport for a liquid to flow a given distance inside a 3D microchannel has been a dream of microfluidic scientists for a long time. A new self-activated pumping mechanism has been proposed in this paper. It is different from the authors' previous research which relied on the fluid resistance of a quartz capillary tube or end-blocked gas-permeable silicone or a polydimethylsiloxane (PDMS) wall to automate the flow. In this research, an end-open stretched Teflon tube was utilized for passive transport for the first time. A new fluid transmission mode was adopted with the assistance of a cheaper easily accessible oil mixture to achieve stable continuous flow. Finally, this novel micropump has been applied to real-time continuous-flow polymerase chain reactions (PCRs), with an amplification efficiency similar to that of a commercial PCR cycler instrument.
语种	英语
内容类型	期刊论文
源URL	[http://ir.ciomp.ac.cn/handle/181722/63002]
专题	中国科学院长春光学精密机械与物理研究所
推荐引用方式 GB/T 7714	K.N.Wang,D.Wu,W.M.Wu. A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor[J]. Micromachines,2019,10(10):12.
APA	K.N.Wang,D.Wu,&W.M.Wu.(2019).A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor.Micromachines,10(10),12.
MLA	K.N.Wang,et al."A New Self-Activated Micropumping Mechanism Capable of Continuous-Flow and Real-Time PCR Amplification Inside 3D Spiral Microreactor".Micromachines 10.10(2019):12.