Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions

doi:10.3389/fgene.2021.795706

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions
	Chan, Jiulin 3,4,5,6; Wang, Lu 2,3,4,5,6; Li, Li 2,3,4,5,6; Mu, Kang 2,3,4,5,6; Bushek, David 1; Xu, Yue 3,4,5,6; Guo, Ximing 1; Zhang, Guofan 2,3,4,5,6; Zhang, Linlin 2,3,4,5,6
刊名	FRONTIERS IN GENETICS
	2021-12-03
卷号	12 页码:16
关键词	oyster comparative transcriptomics dermo disease innate immune response host-parasite interaction gene expansion adaptation
DOI	10.3389/fgene.2021.795706
通讯作者	Guo, Ximing(xguo@hsrl.rutgers.edu) ; Zhang, Guofan(gfzhang@qdio.ac.cn) ; Zhang, Linlin(linlinzhang@qdio.ac.cn)
英文摘要	Infectious disease outbreaks are causing widespread declines of marine invertebrates including corals, sea stars, shrimps, and molluscs. Dermo is a lethal infectious disease of the eastern oyster Crassostrea virginica caused by the protist Perkinsus marinus. The Pacific oyster Crassostrea gigas is resistant to Dermo due to differences in the host-parasite interaction that is not well understood. We compared transcriptomic responses to P. marinus challenge in the two oysters at early and late infection stages. Dynamic and orchestrated regulation of large sets of innate immune response genes were observed in both species with remarkably similar patterns for most orthologs, although responses in C. virginica were stronger, suggesting strong or over-reacting immune response could be a cause of host mortality. Between the two species, several key immune response gene families differed in their expansion, sequence variation and/or transcriptional response to P. marinus, reflecting evolutionary divergence in host-parasite interaction. Of note, significant upregulation of inhibitors of apoptosis (IAPs) was observed in resistant C. gigas but not in susceptible C. virginica, suggesting upregulation of IAPs is an active defense mechanism, not a passive response orchestrated by P. marinus. Compared with C. gigas, C. virginica exhibited greater expansion of toll-like receptors (TLRs) and positive selection in P. marinus responsive TLRs. The C1q domain containing proteins (C1qDCs) with the galactose-binding lectin domain that is involved in P. marinus recognition, were only present and significantly upregulated in C. virginica. These results point to previously undescribed differences in host defense genes between the two oyster species that may account for the difference in susceptibility, providing an expanded portrait of the evolutionary dynamics of host-parasite interaction in lophotrochozoans that lack adaptive immunity. Our findings suggest that C. virginica and P. marinus have a history of coevolution and the recent outbreaks may be due to increased virulence of the parasite.
资助项目	Priority Research Program of the Chinese Academy of Sciences[XDB42000000] ; National Natural Science Foundation of China[41976088] ; Key Development Project of Centre for Ocean Mega-Research of Science, Chinese academy of science[COMS2019R01] ; USDA NIFA Hatch project[1009201-NJ32114] ; USDA NIFA Hatch/Animal Health project[1004475-NJ32920]
WOS研究方向	Genetics & Heredity
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000761070300001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/178062]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Guo, Ximing; Zhang, Guofan; Zhang, Linlin
作者单位	1.Rutgers State Univ, Dept Marine & Coastal Sci, Haskin Shellfish Res Lab, Port Norris, NJ 08349 USA 2.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 6.Chinese Acad Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Chan, Jiulin,Wang, Lu,Li, Li,et al. Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions[J]. FRONTIERS IN GENETICS,2021,12:16.
APA	Chan, Jiulin.,Wang, Lu.,Li, Li.,Mu, Kang.,Bushek, David.,...&Zhang, Linlin.(2021).Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions.FRONTIERS IN GENETICS,12,16.
MLA	Chan, Jiulin,et al."Transcriptomic Response to Perkinsus marinus in Two Crassostrea Oysters Reveals Evolutionary Dynamics of Host-Parasite Interactions".FRONTIERS IN GENETICS 12(2021):16.