A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria

doi:10.3390/molecules26247624

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	A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria
	Luo, Jing-Chang 2,3,4; Zhang, Jian 1; Sun, Li 2,3,4
刊名	MOLECULES
	2021-12-01
卷号	26 期号:24 页码:14
关键词	g-type lysozyme shrimp deep-sea hydrothermal vent non-enzymatic antibacterial activity
DOI	10.3390/molecules26247624
通讯作者	Sun, Li(lsun@qdio.ac.cn)
英文摘要	Lysozyme is a key effector molecule of the innate immune system in both vertebrate and invertebrate. It is classified into six types, one of which is the goose-type (g-type). To date, no study on g-type lysozyme in crustacean has been documented. Here, we report the identification and characterization of a g-type lysozyme (named LysG1) from the shrimp inhabiting a deep-sea hydrothermal vent in Manus Basin. LysG1 possesses conserved structural features of g-type lysozymes. The recombinant LysG1 (rLysG1) exhibited no muramidase activity and killed selectively Gram-negative bacteria in a manner that depended on temperature, pH, and metal ions. rLysG1 bound target bacteria via interaction with bacterial cell wall components, notably lipopolysaccharide (LPS), and induced cellular membrane permeabilization, which eventually caused cell lysis. The endotoxin-binding capacity enabled rLysG1 to alleviate the inflammatory response induced by LPS. Mutation analysis showed that the bacterial binding and killing activities of rLysG1 required the integrity of the conserved alpha 3 and 4 helixes of the protein. Together, these results provide the first insight into the activity and working mechanism of g-type lysozyme in crustacean and deep-sea organisms.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050403] ; CAS Key Laboratory of Molecular Virology & Immunology, Institute Pasteur of Shanghai, Chinese Academy of Sciences[KLMVI-OP-202002] ; Taishan Scholar Program of Shandong Province
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
语种	英语
出版者	MDPI
WOS记录号	WOS:000736322800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177623]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Li
作者单位	1.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 2.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Luo, Jing-Chang,Zhang, Jian,Sun, Li. A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria[J]. MOLECULES,2021,26(24):14.
APA	Luo, Jing-Chang,Zhang, Jian,&Sun, Li.(2021).A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria.MOLECULES,26(24),14.
MLA	Luo, Jing-Chang,et al."A g-Type Lysozyme from Deep-Sea Hydrothermal Vent Shrimp Kills Selectively Gram-Negative Bacteria".MOLECULES 26.24(2021):14.