The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas

doi:10.1016/j.dci.2021.104145

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	The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas
	Xin, Lusheng 3,4; Liu, Chang 1,2,5; Zhang, Huan 3; Qiu, Limei 3; Wang, Lingling 1,2,4,5; Song, Linsheng 1,2,4,5
刊名	DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
	2021-10-01
卷号	123 页码:8
关键词	Crassostrea gigas Cytokine IL-12 IL-12 p35 subunit
ISSN号	0145-305X
DOI	10.1016/j.dci.2021.104145
通讯作者	Song, Linsheng(lshsong@dlou.edu.cn)
英文摘要	Vertebrate interleukin-12 (IL-12) is a heterodimeric cytokine composing of two subunits (p35 and p40). In the present study, a p35-like subunit homolog of vertebrate IL-12 was identified from oyster Crassostrea gigas (designated as CgIL12p35L), with an open reading frame of 411 bp encoding a putative peptide of 136 amino acids. There was a long four-helix chain in CgIL12p35L, which was similar as that in vertebrate IL-12 p35. Comparative genomic analysis showed that there were conservative kinds of syntenic genes flanked CgIL12p35L. The mRNA transcripts of CgIL12p35L were constitutively expressed in various tissues and its mRNA expression level in haemocytes increased significantly after bacteria challenge. The activity of haemolymph to eliminate bacteria from the oysters treated with recombinant CgIL12p35L protein (rCgIL12p35L) in vivo increased significantly. The results collectively indicated that the homolog of vertebrate IL-12 p35 subunit existed in oysters, and it was involved in immune defense against bacteria challenge.
资助项目	National Science Foundation of China[U1706204] ; National Key RD Program[2018YFD0900504] ; China Agriculture Research System of MOF and MARA ; Outstanding Talents and Innovative teams of Agricultural Scientific Research in Ministry of Agriculture ; Research Foundation for Distinguished Professor in Liaoning[XLYC1902012] ; Research Foundation for Climbing Scholars in Liaoning and Talented Scholars in Dalian Ocean University
WOS研究方向	Fisheries ; Immunology ; Veterinary Sciences ; Zoology
语种	英语
出版者	ELSEVIER SCI LTD
WOS记录号	WOS:000672560600004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/177026]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Song, Linsheng
作者单位	1.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian 116023, Peoples R China 2.Dalian Ocean Univ, Dalian Key Lab Aquat Anim Dis Prevent & Control, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266235, Peoples R China 5.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Xin, Lusheng,Liu, Chang,Zhang, Huan,et al. The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2021,123:8.
APA	Xin, Lusheng,Liu, Chang,Zhang, Huan,Qiu, Limei,Wang, Lingling,&Song, Linsheng.(2021).The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,123,8.
MLA	Xin, Lusheng,et al."The characterization of an interleukin-12 p35 homolog involved in the immune modulation of oyster Crassostrea gigas".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 123(2021):8.