Multifunctional alkalophilic alpha-amylase with diverse raw seaweed degrading activities

doi:10.1186/s13568-021-01300-x

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	Multifunctional alkalophilic alpha-amylase with diverse raw seaweed degrading activities
	Gu, Xiaoqian 1,2,5; Fu, Liping 2; Pan, Aihong 2; Gui, Yuanyuan 4; Zhang, Qian 2; Li, Jiang 1,2,3,5
刊名	AMB EXPRESS
	2021-10-20
卷号	11 期号:1 页码:10
关键词	Metagenomic alpha-amylase Multifunctional enzyme Enzymatic digestion
ISSN号	2191-0855
DOI	10.1186/s13568-021-01300-x
通讯作者	Li, Jiang(lijiang@fio.org.cn)
英文摘要	Uncultured microbes are an important resource for the discovery of novel enzymes. In this study, an amylase gene (amy2587) that codes a protein with 587 amino acids (Amy2587) was obtained from the metagenomic library of macroalgae-associated bacteria. Recombinant Amy2587 was expressed in Escherichia coli BL21 (DE3) and was found to simultaneously possess alpha-amylase, agarase, carrageenase, cellulase, and alginate lyase activities. Moreover, recombinant Amy2587 showed high thermostability and alkali resistance which are important characteristics for industrial application. To investigate the multifunctional mechanism of Amy2587, three motifs (functional domains) in the Amy2587 sequence were deleted to generate three truncated Amy2587 variants. The results showed that, even though these functional domains affected the multiple substrates degrading activity of Amy2587, they did not wholly explain its multifunctional characteristics. To apply the multifunctional activity of Amy2587, three seaweed substrates (Grateloupia filicina, Chondrus ocellatus, and Scagassum) were digested using Amy2587. After 2 h, 6 h, and 24 h of digestion, 121.2 +/- 4 mu g/ml, 134.8 +/- 6 mu g/ml, and 70.3 +/- 3.5 mu g/ml of reducing sugars were released, respectively. These results show that Amy2587 directly and effectively degraded three kinds of raw seaweeds. This finding provides a theoretical basis for one-step enzymatic digestion of raw seaweeds to obtain seaweed oligosaccharides.
资助项目	Key Research and Development Program of Shandong Province, China[2018GHY115013] ; Impact and Response of Antarctic Seas to Climate Change[RFSOCC2020-2022]
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000709340500001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/176764]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Li, Jiang
作者单位	1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Minist Nat Resources, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China 3.SOA, Inst Oceanog 1, Key Lab Ecol Environm Sci & Technol, Qingdao 266061, Peoples R China 4.Qingdao Univ, Coll Environm Sci & Engn, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Gu, Xiaoqian,Fu, Liping,Pan, Aihong,et al. Multifunctional alkalophilic alpha-amylase with diverse raw seaweed degrading activities[J]. AMB EXPRESS,2021,11(1):10.
APA	Gu, Xiaoqian,Fu, Liping,Pan, Aihong,Gui, Yuanyuan,Zhang, Qian,&Li, Jiang.(2021).Multifunctional alkalophilic alpha-amylase with diverse raw seaweed degrading activities.AMB EXPRESS,11(1),10.
MLA	Gu, Xiaoqian,et al."Multifunctional alkalophilic alpha-amylase with diverse raw seaweed degrading activities".AMB EXPRESS 11.1(2021):10.