Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*

doi:10.3967/bes2021.074

	Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*
	Jia Rong 1,2,4; Chen Ya Xiong 1,4; Du Ya Rong 1,4; Hu Bu Rong 1,2,3,4
刊名	BIOMEDICAL AND ENVIRONMENTAL SCIENCES
	2021-07-01
卷号	34 期号:7 页码:540-551
关键词	MSD assay Low or high LET irradiation Mouse plasma cytokines Dose estimation model
ISSN号	0895-3988
DOI	10.3967/bes2021.074
通讯作者	Hu Bu Rong(brhu@wmu.edu.cn)
英文摘要	Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer (LET) irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation. Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery (MSD), a high-throughput and sensitive electrochemiluminescence measurement technique. Dose estimation equations were set up using multiple linear regression analysis. Results The relative levels of IL-6 at 1 h, IL-5 and IL-6 at 24 h, and IL-5, IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently. The minimum measured levels of IL-5, IL-6 and IL-15 were up to 4.0076 pg/mL, 16.4538 pg/mL and 0.4150 pg/mL, respectively. In addition, dose estimation models were established and verified. Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5, IL-6 and IL-15. These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators. Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.
资助项目	National Natural Science Foundation of China[11635013] ; National Natural Science Foundation of China[11705248] ; National Natural Science Foundation of China[U1832101] ; National Key Research and Development Program of China[2017YFC0108605] ; Science and Technology Research Project of Gansu Province[145RTSA012] ; Science and Technology Research Project of Gansu Province[17JR5RA307] ; Heavy Ion Research Facility in Lanzhou[Y9HIRFL200]
WOS关键词	CHROMOSOME-ABERRATION ANALYSIS ; MICRONUCLEUS ASSAY ; RADIATION ; BIODOSIMETRY ; EXPOSURE ; ELISA ; MODULATION ; WORKERS ; HUMANS ; CANCER
WOS研究方向	Environmental Sciences & Ecology ; Public, Environmental & Occupational Health
语种	英语
出版者	CHINESE CENTER DISEASE CONTROL & PREVENTION
WOS记录号	WOS:000691530100004
资助机构	National Natural Science Foundation of China ; National Key Research and Development Program of China ; Science and Technology Research Project of Gansu Province ; Heavy Ion Research Facility in Lanzhou
内容类型	期刊论文
源URL	[http://119.78.100.186/handle/113462/136381]
专题	中国科学院近代物理研究所
通讯作者	Hu Bu Rong
作者单位	1.Chinese Acad Sci, CAS Key Lab Heavy Ion Radiat Biol & Med, Inst Modern Phys, Lanzhou 730000, Gansu, Peoples R China 2.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing 100039, Peoples R China 3.Wenzhou Med Univ, Sch Publ Hlth & Management, Dept Radiat Med, Wenzhou 325035, Zhejiang, Peoples R China 4.Chinese Acad Sci, Key Lab Space Radiobiol Gansu Prov, Lanzhou 730000, Gansu, Peoples R China
推荐引用方式 GB/T 7714	Jia Rong,Chen Ya Xiong,Du Ya Rong,et al. Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*[J]. BIOMEDICAL AND ENVIRONMENTAL SCIENCES,2021,34(7):540-551.
APA	Jia Rong,Chen Ya Xiong,Du Ya Rong,&Hu Bu Rong.(2021).Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*.BIOMEDICAL AND ENVIRONMENTAL SCIENCES,34(7),540-551.
MLA	Jia Rong,et al."Meso-scale Discovery Assay Detects the Changes of Plasma Cytokine Levels in Mice after Low or High LET Ionizing Irradiation*".BIOMEDICAL AND ENVIRONMENTAL SCIENCES 34.7(2021):540-551.