Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL

doi:10.1016/j.jplph.2016.12.014

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	Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL
	Suga, Michihiro ; Akita, Fusamichi 13; Sugahara, Michihiro 12; Kubo, Minoru 12,13; Nakajima, Yoshiki ; Nakane, Takanori 11; Yamashita, Keitaro 12; Umena, Yasufumi ; Nakabayashi, Makoto ; Yamane, Takahiro
刊名	NATURE
	2017
卷号	543 期号:7643 页码:131+
ISSN号	0028-0836
DOI	10.1016/j.jplph.2016.12.014
文献子类	Article
英文摘要	Photosystem II (PSII) is a huge membrane-protein complex consisting of 20 different subunits with a total molecular mass of 350 kDa for a monomer. It catalyses light-driven water oxidation at its catalytic centre, the oxygen-evolving complex (OEC)(1-3). The structure of PSII has been analysed at 1.9 angstrom resolution by synchrotron radiation X-rays, which revealed that the OEC is a Mn4CaO5 cluster organized in an asymmetric, `distorted-chair' form(4). This structure was further analysed with femtosecond X-ray free electron lasers (XFEL), providing the `radiation damage-free'(5) structure. The mechanism of O=O bond formation, however, remains obscure owing to the lack of intermediate-state structures. Here we describe the structural changes in PSII induced by two-flash illumination at room temperature at a resolution of 2.35 angstrom using time-resolved serial femtosecond crystallography with an XFEL provided by the SPring-8 angstrom compact free-electron laser. An isomorphous difference Fourier map between the two-flash and dark-adapted states revealed two areas of apparent changes: around the QB/non-haem iron and the Mn4CaO5 cluster. The changes around the QB/non-haem iron region reflected the electron and proton transfers induced by the two-flash illumination. In the region around the OEC, a water molecule located 3.5 angstrom from the Mn4CaO5 cluster disappeared from the map upon two-flash illumination. This reduced the distance between another water molecule and the oxygen atom O4, suggesting that proton transfer also occurred. Importantly, the two-flash-minus-dark isomorphous difference Fourier map showed an apparent positive peak around O5, a unique mu 4-oxo-bridge located in the quasi-centre of Mn1 and Mn4 (refs 4,5). This suggests the insertion of a new oxygen atom (O6) close to O5, providing an O=O distance of 1.5 angstrom between these two oxygen atoms. This provides a mechanism for the O=O bond formation consistent with that proposed previously(6,7.)
学科主题	Plant Sciences
电子版国际标准刊号	1476-4687
出版地	LONDON
WOS关键词	OXYGEN-EVOLVING COMPLEX ; X-RAY-DIFFRACTION ; PHOTOSYNTHETIC WATER OXIDATION ; PHOTOSYSTEM-II ; INFRARED DETECTION ; OXIDIZING COMPLEX ; PROTON RELEASE ; MECHANISM ; STATE ; BINDING
语种	英语
出版者	NATURE PUBLISHING GROUP
WOS记录号	WOS:000401099400010
资助机构	Okayama University ; JSPS KAKENHIMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [JP15H01642, JP16H06162, JP16H06296, JP16K21181, JP15H05588, JP15H03841, JP15H01055, JP24000018] ; MEXT, JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) ; Asahi Glass Foundation ; Kato Memorial Bioscience Foundation ; Inamori Foundation ; Japan Science and Technology agency (JST)Japan Science & Technology Agency (JST) ; PRESTO from JSTJapan Science & Technology Agency (JST) ; Pioneering Project 'Dynamic Structural Biology' of RIKEN ; CASChinese Academy of Sciences [XDB17030100] ; Grants-in-Aid for Scientific ResearchMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of ScienceGrants-in-Aid for Scientific Research (KAKENHI) [16H06162, 26440028] Funding Source: KAKEN
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22011]
专题	中科院光生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 2.Kyoto Univ, Grad Sch Med, Dept Cell Biol, Sakyo Ku, Yoshidakonoe Cho, Kyoto 6068501, Japan 3.Japan Synchrotron Radiat Res Inst, 1-1-1 Kouto, Sayo, Hyogo 6795198, Japan 4.Nagoya Univ, Grad Sch Sci, Div Mat Sci, Chikusa Ku, Furo Cho, Nagoya, Aichi 4648602, Japan 5.Univ Hyogo, Grad Sch Life Sci, Dept Picobiol, 3-2-1 Kouto,Kamigori Cho, Kobe, Hyogo 6781297, Japan 6.Kobe Univ, Grad Sch Sci, Dept Chem, Nada Ku, 1-1 Rokkodai, Kobe, Hyogo 6578501, Japan 7.Univ Tokyo, Grad Sch Med, Dept Cell Biol & Anat, Bunkyo Ku, Hongo, Tokyo 1130033, Japan 8.Kyoto Univ, Grad Sch Agr, Div Food Sci & Biotechnol, Uji, Kyoto 6110011, Japan 9.Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan 10.Univ Tokyo, Grad Sch Sci, Dept Biol Sci, Bunkyo Ku, 2-11-16 Yayoi, Tokyo 1130032, Japan
推荐引用方式 GB/T 7714	Suga, Michihiro,Akita, Fusamichi,Sugahara, Michihiro,et al. Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL[J]. NATURE,2017,543(7643):131+.
APA	Suga, Michihiro.,Akita, Fusamichi.,Sugahara, Michihiro.,Kubo, Minoru.,Nakajima, Yoshiki.,...&Shen, Jian-Ren.(2017).Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL.NATURE,543(7643),131+.
MLA	Suga, Michihiro,et al."Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL".NATURE 543.7643(2017):131+.