Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile

doi:10.1007/s11120-018-0557-1

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	Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile
	Streckaite, Simona ; Gardian, Zdenko 1,2; Li, Fei 3; Pascal, Andrew A.; Litvin, Radek 1,2; Robert, Bruno ; Llansola-Portoles, Manuel J.
刊名	PHOTOSYNTHESIS RESEARCH
	2018
卷号	138 期号:2 页码:139-148
关键词	Light-harvesting complex Algae Resonance Raman Chl-a Carotenoids Diadinoxanthin Heteroxanthin
ISSN号	0166-8595
DOI	10.1007/s11120-018-0557-1
文献子类	Article
英文摘要	The soil chromophyte alga Xanthonema (X.) debile contains only non-carbonyl carotenoids and Chl-a. X. debile has an antenna system denoted Xanthophyte light-harvesting complex (XLH) that contains the carotenoids diadinoxanthin, heteroxanthin, and vaucheriaxanthin. The XLH pigment stoichiometry was calculated by chromatographic techniques and the pigment-binding structure studied by resonance Raman spectroscopy. The pigment ratio obtained by HPLC was found to be close to 8:1:2:1 Chl-a:heteroxanthin:diadinoxanthin:vaucheriaxanthin. The resonance Raman spectra suggest the presence of 8-10 Chl-a, all of which are 5-coordinated to the central Mg, with 1-3 Chl-a possessing a macrocycle distorted from the relaxed conformation. The three populations of carotenoids are in the all-trans configuration. Vaucheriaxanthin absorbs around 500-530nm, diadinoxanthin at 494nm and heteroxanthin at 487nm at 4.5K. The effective conjugation length of heteroxanthin and diadinoxanthin has been determined as 9.4 in both cases; the environment polarizability of the heteroxanthin and diadinoxanthin binding pockets is 0.270 and 0.305, respectively.
学科主题	Plant Sciences
电子版国际标准刊号	1573-5079
出版地	DORDRECHT
WOS关键词	RESONANCE RAMAN-SPECTRA ; FUCOXANTHIN-CHLOROPHYLL-PROTEIN ; EXCITATION-ENERGY TRANSFER ; CIS-TRANS ISOMERS ; BETA-CAROTENE ; PHOTOSYNTHETIC BACTERIA ; ELECTRONIC ABSORPTION ; INFRARED-SPECTRA ; REACTION CENTERS ; ALL-TRANS
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000448512400002
资助机构	ERC funding agency (PHOTPROT project) ; French Infrastructure for Integrated Structural Biology (FRISBI) [ANR-10-INBS-05] ; European Union 2020 research and innovation program under the Marie Sklodowska-Curie Grant [675006 (SE2B)] ; Czech Science FoundationGrant Agency of the Czech Republic [P501/12/G055] ; European Regional Development FundEuropean Commission ; [RVO:60077344]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/20675]
专题	中科院光生物学重点实验室
作者单位	1.[Streckaite, Simona; Li, Fei; Pascal, Andrew A.; Robert, Bruno; Llansola-Portoles, Manuel J.] Univ Paris Saclay, CEA, CNRS, Inst Integrat Biol Cell I2BC, F-91198 Gif Sur Yvette, France 2.Czech Acad Sci, Biol Ctr, Branisovska 31, Ceske Budejovice 37005, Czech Republic 3.Univ South Bohemia, Fac Sci, Branisovska 1760, Ceske Budejovice 37005, Czech Republic 4.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Streckaite, Simona,Gardian, Zdenko,Li, Fei,et al. Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile[J]. PHOTOSYNTHESIS RESEARCH,2018,138(2):139-148.
APA	Streckaite, Simona.,Gardian, Zdenko.,Li, Fei.,Pascal, Andrew A..,Litvin, Radek.,...&Llansola-Portoles, Manuel J..(2018).Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile.PHOTOSYNTHESIS RESEARCH,138(2),139-148.
MLA	Streckaite, Simona,et al."Pigment configuration in the light-harvesting protein of the xanthophyte alga Xanthonema debile".PHOTOSYNTHESIS RESEARCH 138.2(2018):139-148.