Identification and characterization of Rubisco activase genes in Oryza punctata

doi:10.1080/13658816.2016.1195502

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	Identification and characterization of Rubisco activase genes in Oryza punctata
	Xu, Si ; Qin, Zong-Yan ; Gong, Pi-Chang ; Dong, Qian-Li ; Bao, Ying 5
刊名	JOURNAL OF SYSTEMATICS AND EVOLUTION
	2017
卷号	55 期号:3 页码:200-207
关键词	Rubisco activase alternative splicing wild rice expression divergence protein interaction
ISSN号	1674-4918
DOI	10.1080/13658816.2016.1195502
文献子类	Article
英文摘要	Rubisco activase (Rca), a specific chaperone, catalyzes the in vivo activation of Rubisco, and thus plays a major role in plant photosynthesis. Although the genes encoding Rubisco activase have been studied in many model or economic plants, few studies have analyzed their homologs in plants of closely related crop species. In this study, an Rca gene was identified and characterized in a wild relative of rice, Oryza punctata Kotschy ex Steud. The gene was 2747 bp long and possessed six exons and five introns with 47% GC content. Furthermore, cDNA sequencing produced two transcripts, RcaL and RcaS, that differed in the sequence by an inclusion of 99 bp at the carboxy terminus of RcaL. Sequence comparison between the two transcripts and the genomic DNA showed that there was a 20-bp alternative splicing event that occurred at the fifth intron of the gene leading to the synthesis of a short polypeptide. The leaf transcriptome analysis showed that RcaS had a higher expression level than that of RcaL in a normal growth environment. In addition, the yeast two-hybrid assays showed the small isoform of Rubisco activase in close contact with the large subunit of the Rubisco in this species, supporting the side-on binding model of interaction between two proteins. This study broadens our understanding of the molecular characteristics of some essential genes in photosynthesis.
学科主题	Computer Science ; Geography ; Physical Geography ; Information Science & Library Science
电子版国际标准刊号	1759-6831
出版地	HOBOKEN
WOS关键词	RIBULOSE 1,5-BISPHOSPHATE ; CARBOXYLASE/OXYGENASE ACTIVASE ; EXPRESSION ; PLANTS ; PHOTOSYNTHESIS ; SUBSTITUTION ; ALIGNMENT ; ISOFORMS ; ENZYMES ; MAIZE
语种	英语
出版者	WILEY
WOS记录号	WOS:000387268600010
资助机构	National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570218]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/22074]
专题	系统与进化植物学国家重点实验室
作者单位	1.Northeast Normal Univ, Key Lab Mol Epigenet, Minist Educ, Changchun 130024, Peoples R China 2.[Xu, Si 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Qufu Normal Univ, Sch Life Sci, Qufu 273165, Shandong, Peoples R China 5.Qin, Zong-Yan
推荐引用方式 GB/T 7714	Xu, Si,Qin, Zong-Yan,Gong, Pi-Chang,et al. Identification and characterization of Rubisco activase genes in Oryza punctata[J]. JOURNAL OF SYSTEMATICS AND EVOLUTION,2017,55(3):200-207.
APA	Xu, Si,Qin, Zong-Yan,Gong, Pi-Chang,Dong, Qian-Li,&Bao, Ying.(2017).Identification and characterization of Rubisco activase genes in Oryza punctata.JOURNAL OF SYSTEMATICS AND EVOLUTION,55(3),200-207.
MLA	Xu, Si,et al."Identification and characterization of Rubisco activase genes in Oryza punctata".JOURNAL OF SYSTEMATICS AND EVOLUTION 55.3(2017):200-207.