Diatom DNA barcodes for forensic discrimination of drowning incidents

doi:10.1093/femsle/fnaa145

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	Diatom DNA barcodes for forensic discrimination of drowning incidents
	Liu, Mengyan 1; Zhao, Yi 1; Sun, Yuzhe 2,3; Wu, Ping 2,3; Zhou, Shiliang 2,3; Ren, Liang
刊名	FEMS MICROBIOLOGY LETTERS
	2020
卷号	367 期号:17
关键词	18S diatom DNA barcode primer rbcL
ISSN号	0378-1097
DOI	10.1093/femsle/fnaa145
文献子类	Article
英文摘要	The presence of diatoms in victim's internal organs has been regarded as a gold biological evidence of drowning. The idea becomes true at the advent of DNA metabarcoding. Unfortunately, the DNA barcode of diatoms are far from being applicable due to neither consensus on the barcode and nor reliable reference library.In this study we tested 23 pairs of primers, including two new primer pairs, Baci18S (V4 of 18S) and BacirbcL (central region of rbcL), for amplifying fragments of 16S/18S, 23S/28S, COI, ITS and rbcL. A total of five pairs of primers performed satisfactory for diatoms. We used three of them, 18S605 (V2 + V3 of 18S), Baci18S and BacirbcL, to barcode four water samples using next generation sequencing platform. The results showed that these primers worked well for NGS metabarcoding of diatoms. We suggest that 18S605, Baci18S and BacirbcL be barcodes of diatoms and the corresponding primer pairs be used. Considering a quite high proportion of sequences deposited in GenBank were mislabeled, the most urgent task for DNA barcoding of diatoms is to create standard sequences using correctly identified specimens, ideally type specimens.
学科主题	Microbiology
电子版国际标准刊号	1574-6968
出版地	OXFORD
WOS关键词	IDENTIFICATION ; SOFTWARE ; RBCL
WOS研究方向	Microbiology
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000602578400008
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/21487]
专题	系统与进化植物学国家重点实验室
作者单位	1.Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Forens Med, 13 HangkongRd, Wuhan 430030, Hubei, Peoples R China 2.Beijing Publ Secur Bur, Forens Judicial Appraisal Ctr, 1 Longgang Beijing Rd, Beijing 100192, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Xiangshan St, Beijing 100093, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Mengyan,Zhao, Yi,Sun, Yuzhe,et al. Diatom DNA barcodes for forensic discrimination of drowning incidents[J]. FEMS MICROBIOLOGY LETTERS,2020,367(17).
APA	Liu, Mengyan,Zhao, Yi,Sun, Yuzhe,Wu, Ping,Zhou, Shiliang,&Ren, Liang.(2020).Diatom DNA barcodes for forensic discrimination of drowning incidents.FEMS MICROBIOLOGY LETTERS,367(17).
MLA	Liu, Mengyan,et al."Diatom DNA barcodes for forensic discrimination of drowning incidents".FEMS MICROBIOLOGY LETTERS 367.17(2020).