Adaptation evolution and bioactivity of galectin from the deep sea Vesicomyidae clam Archivesica packardana

doi:10.1016/j.fsi.2019.12.064

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	Adaptation evolution and bioactivity of galectin from the deep sea Vesicomyidae clam Archivesica packardana
	Kong, Xue 1,2; Li, Yanan 1,2; Zhang, Haibin 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2020-02-01
卷号	97 页码:483-492
关键词	Innate immunity Cold seep Archivesica packardana Galectin Adaptation evolution
ISSN号	1050-4648
DOI	10.1016/j.fsi.2019.12.064
英文摘要	Hydrothermal vents and cold seep zones are two special habitats in the deep sea. These habitats are always dark, and have extreme temperatures (low or high), heavy metals and toxic substances (sulfide, methane). Vesicomyidae clams, which maintain endosymbionts in their gills, are common species in these two special zones and are thought to develop an efficacious immune system against unusual habitats. In the present study, a novel galectin (Apgalectin) was identified from the Vesicomyidae clam Archivesica packardana. The phylogenetic tree indicated that Apgalectin had two CRDs and was closely clustered with galectins from invertebrates, especially mollusks. A branch-site model showed that 9 positively selected sites (omega 2 = 6.83950) were identified comparing to galectins from the Order Veneroida, implying a different function of Vesicomyidae galectins. A microbe binding assay showed that rApgalectin could bind to gram-positive bacteria, gram-negative bacteria and fungi. A PAMP binding assay indicated that Apgalectin could bind LPS, PGN, beta-1,3-glucan, glucan from yeast and Poly I:C in dose-dependent manner. Apgalectin only agglutinated Micrococcus luteus and agglutination could be inhibited by galactose which demonstrated that Apgalectin might be involved in immune defense by recognizing and binding bacteria in a beta-galactoside manner. Further experiments showed that Apgalectin might play an indirect effector role in the immune response because of its limited antibacterial spectrum. All analyses validated that Apgalectin from Archivesica packardana plays a variety of functions in immune responses and provided basal information for the immune study of deep-sea mollusks.
资助项目	Strategic Priority Research Program of the Chinese Academy of Sciences (CAS)[XDA22050303] ; National Natural Science Foundation of China[41576127] ; National Key Research and Development Program of China[2016YFC0304905] ; Knowledge Innovation Program of CAS[SIDSSE-201401]
WOS关键词	C-TYPE LECTIN ; PEPTIDOGLYCAN RECOGNITION PROTEIN ; MUSSEL BATHYMODIOLUS-AZORICUS ; SHRIMP LITOPENAEUS-VANNAMEI ; TANDEM-REPEAT GALECTIN ; MOLECULAR DIVERSITY ; CHLAMYS-FARRERI ; EXPRESSION ; RECEPTOR ; DOMAIN
WOS研究方向	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
WOS记录号	WOS:000513983700050
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) ; National Natural Science Foundation of China ; National Key Research and Development Program of China ; Knowledge Innovation Program of CAS
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/7471]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin
作者单位	1.Chinese Acad Sci, Inst Deep Sea Sci & Engn, Sanya 572000, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Kong, Xue,Li, Yanan,Zhang, Haibin. Adaptation evolution and bioactivity of galectin from the deep sea Vesicomyidae clam Archivesica packardana[J]. FISH & SHELLFISH IMMUNOLOGY,2020,97:483-492.
APA	Kong, Xue,Li, Yanan,&Zhang, Haibin.(2020).Adaptation evolution and bioactivity of galectin from the deep sea Vesicomyidae clam Archivesica packardana.FISH & SHELLFISH IMMUNOLOGY,97,483-492.
MLA	Kong, Xue,et al."Adaptation evolution and bioactivity of galectin from the deep sea Vesicomyidae clam Archivesica packardana".FISH & SHELLFISH IMMUNOLOGY 97(2020):483-492.