Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain
	Guo, Zhiling; Zhang, Peng; Luo, Yali; Xie, Heidi Qunhui; Chakraborty, Swaroop; Monikh, Fazel Abdolahpur; Bu, Lijing; Liu, Yiyun; Ma, Yongchao; Zhang, Zhiyong
刊名	NANO TODAY
	2020-12
卷号	35 页码:1-8
关键词	Zinc oxide nanoparticles Brain Cholinergic neurotransmission Acetylcholine Acetylcholinesterase
ISSN号	1748-0132
英文摘要	The neurotoxicity of inhaled ZnO nanoparticles (NPs) and the underlying mechanisms remain largely unknown. In this study, ZnO NPs (30 +/- 6 nm) were intranasally instilled to rats via a single dose (13 mg Zn/kg BW), with ZnSO4 as the ionic control, and analysis 7-days post exposure. The hippocampus was found to be the main target for Zn accumulation for both ZnO NPs and ZnSO4. Synchrotron radiation based X-ray absorption fine structure (XAFS) analysis showed that no particulate ZnO was found, suggesting the occurrence of dissolution and transformation of ZnO NPs. Multi-omics analysis, including transcriptomics, proteomics and metabolomics, demonstrated that cholinergic neurotransmission was the main biological process affected following both treatments. The release of the key neurotransmitter acetylcholine (ACh) was increased by enhanced ACh synthesis, upregulation of vesicular ACh transporter, and suppression of the activity of ACh hydrolysis enzyme (AChE), either by direct Zn-AChE interaction or a transcriptional down-regulation mechanism. In addition, ZnO NPs and ZnSO4 induced similar molecular consequences and exhibited the same Zn chemical speciation (100 % of Zn complexes) in the hippocampal region evidenced by XAFS analysis, suggesting that the observed biological effects were mainly derived from Zn2+ released from the ZnO NPs. This study not only evidences a new pathway for the impact of ZnO NPs on the brain, but also identifies the origin of the impact as ionic Zn, which provides the basis for safe-by-design of ZnO NPs. (C) 2020 The Author(s). Published by Elsevier Ltd.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/44716]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Guo, Zhiling,Zhang, Peng,Luo, Yali,et al. Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain[J]. NANO TODAY,2020,35:1-8.
APA	Guo, Zhiling.,Zhang, Peng.,Luo, Yali.,Xie, Heidi Qunhui.,Chakraborty, Swaroop.,...&Lynch, Iseult.(2020).Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain.NANO TODAY,35,1-8.
MLA	Guo, Zhiling,et al."Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain".NANO TODAY 35(2020):1-8.