Radiopharmaceutical and Eu3+ doped gadolinium oxide nanoparticles mediated triple-excited fluorescence imaging and image-guided surgery

doi:10.1186/s12951-021-00920-6

CORC > 自动化研究所 > 中国科学院自动化研究所 > 中国科学院分子影像重点实验室

	Radiopharmaceutical and Eu3+ doped gadolinium oxide nanoparticles mediated triple-excited fluorescence imaging and image-guided surgery
	Shi,Xiaojing 1,2; Cao,Caiguang 1,2; Zhang,Zeyu 1,3; Tian,Jie 1,2,3; Hu,Zhenhua 1,2
刊名	Journal of Nanobiotechnology
	2021-07-16
卷号	19 期号:1 页码:14
关键词	Radiopharmaceuticals Gd2O3:Eu Cerenkov luminescence imaging Optical imaging Image-guided surgery
DOI	10.1186/s12951-021-00920-6
通讯作者	Tian,Jie(jie.tian@ia.ac.cn) ; Hu,Zhenhua(zhenhua.hu@ia.ac.cn)
英文摘要	AbstractCerenkov luminescence imaging (CLI) is a novel optical imaging technique that has been applied in clinic using various radionuclides and radiopharmaceuticals. However, clinical application of CLI has been limited by weak optical signal and restricted tissue penetration depth. Various fluorescent probes have been combined with radiopharmaceuticals for improved imaging performances. However, as most of these probes only interact with Cerenkov luminescence (CL), the low photon fluence of CL greatly restricted it’s interaction with fluorescent probes for in vivo imaging. Therefore, it is important to develop probes that can effectively convert energy beyond CL such as β and γ to the low energy optical signals. In this study, a Eu3+ doped gadolinium oxide (Gd2O3:Eu) was synthesized and combined with radiopharmaceuticals to achieve a red-shifted optical spectrum with less tissue scattering and enhanced optical signal intensity in this study. The interaction between Gd2O3:Eu and radiopharmaceutical were investigated using 18F-fluorodeoxyglucose (18F-FDG). The ex vivo optical signal intensity of the mixture of Gd2O3:Eu and 18F-FDG reached 369 times as high as that of CLI using 18F-FDG alone. To achieve improved biocompatibility, the Gd2O3:Eu nanoparticles were then modified with polyvinyl alcohol (PVA), and the resulted nanoprobe PVA modified Gd2O3:Eu (Gd2O3:Eu@PVA) was applied in intraoperative tumor imaging. Compared with 18F-FDG alone, intraoperative administration of Gd2O3:Eu@PVA and 18F-FDG combination achieved a much higher tumor-to-normal tissue ratio (TNR, 10.24?±?2.24 vs. 1.87?±?0.73, P?=?0.0030). The use of Gd2O3:Eu@PVA and 18F-FDG also assisted intraoperative detection of tumors that were omitted by preoperative positron emission tomography (PET) imaging. Further experiment of image-guided surgery demonstrated feasibility of image-guided tumor resection using Gd2O3:Eu@PVA and 18F-FDG. In summary, Gd2O3:Eu can achieve significantly optimized imaging property when combined with 18F-FDG in intraoperative tumor imaging and image-guided tumor resection surgery. It is expected that the development of the Gd2O3:Eu nanoparticle will promote investigation and application of novel nanoparticles that can interact with radiopharmaceuticals for improved imaging properties. This work highlighted the impact of the nanoprobe that can be excited by radiopharmaceuticals emitting CL, β, and γ radiation for precisely imaging of tumor and intraoperatively guide tumor resection.
资助项目	National Key Research and Development Program of China[2017YFA0205200] ; National Natural Science Foundation of China (NSFC)[62027901] ; National Natural Science Foundation of China (NSFC)[81930053] ; National Natural Science Foundation of China (NSFC)[92059207] ; National Natural Science Foundation of China (NSFC)[81227901] ; Beijing Natural Science Foundation[JQ19027] ; innovative research team of high-level local universities in Shanghai ; Zhuhai High-level Health Personnel Team Project[HLHPTP201703]
WOS关键词	LUMINESCENCE ; RADIATION ; THERAPY
WOS研究方向	Biotechnology & Applied Microbiology ; Science & Technology - Other Topics
语种	英语
出版者	BioMed Central
WOS记录号	BMC:10.1186/S12951-021-00920-6
资助机构	National Key Research and Development Program of China ; National Natural Science Foundation of China (NSFC) ; Beijing Natural Science Foundation ; innovative research team of high-level local universities in Shanghai ; Zhuhai High-level Health Personnel Team Project
内容类型	期刊论文
源URL	[http://ir.ia.ac.cn/handle/173211/45087]
专题	自动化研究所_中国科学院分子影像重点实验室
通讯作者	Tian,Jie; Hu,Zhenhua
作者单位	1.Chinese Academy of Sciences; CAS Key Laboratory of Molecular Imaging, Beijing Key Laboratory of Molecular Imaging, The State Key Laboratory of Management and Control for Complex Systems, Institute of Automation 2.University of Chinese Academy of Sciences; School of Artificial Intelligence 3.Beihang University; Beijing Advanced Innovation Center for Big Data-Based Precision Medicine, School of Medicine
推荐引用方式 GB/T 7714	Shi,Xiaojing,Cao,Caiguang,Zhang,Zeyu,et al. Radiopharmaceutical and Eu3+ doped gadolinium oxide nanoparticles mediated triple-excited fluorescence imaging and image-guided surgery[J]. Journal of Nanobiotechnology,2021,19(1):14.
APA	Shi,Xiaojing,Cao,Caiguang,Zhang,Zeyu,Tian,Jie,&Hu,Zhenhua.(2021).Radiopharmaceutical and Eu3+ doped gadolinium oxide nanoparticles mediated triple-excited fluorescence imaging and image-guided surgery.Journal of Nanobiotechnology,19(1),14.
MLA	Shi,Xiaojing,et al."Radiopharmaceutical and Eu3+ doped gadolinium oxide nanoparticles mediated triple-excited fluorescence imaging and image-guided surgery".Journal of Nanobiotechnology 19.1(2021):14.