Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles

doi:10.1016/j.jgg.2021.03.004

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	Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles
	Jiao, Shuang 1,2,3; Xu, Rui 1; Du, Shaojun 1
刊名	JOURNAL OF GENETICS AND GENOMICS
	2021-03-20
卷号	48 期号:3 页码:208-218
关键词	Smyd1 Myosin Sarcomere Craniofacial muscle Cardiac muscle
ISSN号	1673-8527
DOI	10.1016/j.jgg.2021.03.004
通讯作者	Du, Shaojun(sdu@som.umaryland.edu)
英文摘要	Skeletal and cardiac muscles are striated myofibers that contain highly organized sarcomeres for muscle contraction. Recent studies revealed that Smyd1, a lysine methyltransferase, plays a key role in sarcomere assembly in heart and trunk skeletal muscles. However, Smyd1 expression and function in craniofacial muscles are not known. Here, we analyze the developmental expression and function of two smyd1 paralogous genes, smyd1a and smyd1b, in craniofacial and cardiac muscles of zebrafish embryos. Our data show that loss of smyd1a (smyd1a(mb5)) or smyd1b (smyd1b(sa15678)) has no visible effects on myogenic commitment and expression of myod and myosin heavy-chain mRNA transcripts in craniofacial muscles. However, myosin heavy-chain protein accumulation and sarcomere organization are dramatically reduced in smyd1b(sa15678) single mutant, and almost completely diminish in smyd1a(mb5); smyd1b(sa15678) double mutant, but not in smyd1a(mb5) mutant. Similar defects are also observed in cardiac muscles of smyd1b(sa15678) mutant. Defective craniofacial and cardiac muscle formation is associated with an upregulation of hsp90 alpha 1 and unc45b mRNA expression in smyd1b(sa15678) and smyd1a(mb5); smyd1b(sa15678) mutants. Together, our studies indicate that Smyd1b, but not Smyd1a, plays a key role in myosin heavy-chain protein expression and sarcomere organization in craniofacial and cardiac muscles. Loss of smyd1b results in muscle-specific stress response. Copyright (C) 2021, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press. All rights reserved.
资助项目	U.S. National Institute of Health (NIH) National Institute of Arthritis and Musculoskeletal and Skin Diseases[R01AR072703] ; China Scholarship Council
WOS研究方向	Biochemistry & Molecular Biology ; Genetics & Heredity
语种	英语
出版者	SCIENCE PRESS
WOS记录号	WOS:000681047800004
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/175989]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Du, Shaojun
作者单位	1.Univ Maryland, Inst Marine & Environm Technol, Sch Med, Dept Biochem & Mol Biol, Baltimore, MD 21202 USA 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
推荐引用方式 GB/T 7714	Jiao, Shuang,Xu, Rui,Du, Shaojun. Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles[J]. JOURNAL OF GENETICS AND GENOMICS,2021,48(3):208-218.
APA	Jiao, Shuang,Xu, Rui,&Du, Shaojun.(2021).Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles.JOURNAL OF GENETICS AND GENOMICS,48(3),208-218.
MLA	Jiao, Shuang,et al."Smyd1 is essential for myosin expression and sarcomere organization in craniofacial, extraocular, and cardiac muscles".JOURNAL OF GENETICS AND GENOMICS 48.3(2021):208-218.