Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum

doi:10.1016/j.ecoenv.2021.112528

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室 > 近岸生态与环境实验室

	Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum
	Cong, Ming 1; Li, Yuanmei 1; Xu, Hongchao 2; Lv, Jiasen 2; Wu, Huifeng 3; Zhao, Ye 1
刊名	ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
	2021-10-01
卷号	222 页码:7
关键词	Ammonia nitrogen Gill mitochondria Swelling MtDNA copy Succinic dehydrogenase Mitochondrial ultrastructure
ISSN号	0147-6513
DOI	10.1016/j.ecoenv.2021.112528
通讯作者	Lv, Jiasen(lvjiasencong@163.com) ; Wu, Huifeng(hfwu@yic.ac.cn)
英文摘要	Ammonia nitrogen has been one of the key pollution indicators along the Chinese coastline for quite a few years. Our previous studies have proved that ammonia nitrogen is harmful for Ruditapes philippinarum clam in several aspects. Environmental concentrations of ammonia nitrogen were found to significantly decrease ATP contents and disturb ATP metabolism, in addition to reducing the potential across the mitochondrial membrane in clam gill tissues. Accordingly, mitochondrion is considered as one of the target organelles of ammonia nitrogen toxicity in clams. However, there is a lack of direct evidence to prove it. In order to reveal detail information of ammonia nitrogen toxicity on clam mitochondria and screen the related biomarker to indicate ammonia nitrogen pollution, mitochondrial parameters in gill tissues including swelling, mtDNA copy number and marker enzyme (succinic dehydrogenase, SDH) activity were measured after the clams were exposed to 0.1 mg/L and 0.5 mg/L ammonia nitrogen for 3 days and 21 days, respectively. Moreover, adverse effects of ammonia nitrogen exposure on clam mitochondrial ultra-structures, mitochondrial swelling and division were also discriminated under transmission electron microscope (TEM). Final results showed that ammonia nitrogen exposure to both concentrations significantly induced mitochondrial swelling, reduced the number of mitochondria and messed their normal structure, decreased the number of mtDNA copies and down-regulated SDH activity, all in a concentration and duration dependent manner. So, the present study helps us to better understand the structural damage of ammonia nitrogen on mitochondria in clam gill cells and provides fundamental data for ammonia nitrogen control in aquaculture.
资助项目	Natural Science Foundation of China[NSFC 41876135]
WOS关键词	DEHYDROGENASE ACTIVITY ; TOXICITY ; MTDNA
WOS研究方向	Environmental Sciences & Ecology ; Toxicology
语种	英语
WOS记录号	WOS:000687402400006
资助机构	Natural Science Foundation of China
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/29727]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	Lv, Jiasen; Wu, Huifeng
作者单位	1.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China 2.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Cong, Ming,Li, Yuanmei,Xu, Hongchao,et al. Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2021,222:7.
APA	Cong, Ming,Li, Yuanmei,Xu, Hongchao,Lv, Jiasen,Wu, Huifeng,&Zhao, Ye.(2021).Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,222,7.
MLA	Cong, Ming,et al."Ammonia nitrogen exposure caused structural damages to gill mitochondria of clam Ruditapes philippinarum".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 222(2021):7.