Factors Affecting the Catalytic Efficiency and Synergism of Xylanase and Cellulase During Enzymatic Hydrolysis of Birch Wood

doi:10.1007/s12010-021-03590-0

	Factors Affecting the Catalytic Efficiency and Synergism of Xylanase and Cellulase During Enzymatic Hydrolysis of Birch Wood
	Li, Hailong 1,2,4,6; Chen, Xindong 3,4; Wang, Can 1,2,4,6; Chen, Xuefang 1,2,4,6; Guo, Haijun 1,2,4,6; Xiong, Lian 1,2,4,6; Zhang, Hairong 1,2,4,6; Huang, Chao 5; Chen, Xinde 1,2,4,6
刊名	APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
	2021-07-10
页码	14
关键词	Delignification Deacetylation Decrystallization Synergism Enzymatic hydrolysis
ISSN号	0273-2289
DOI	10.1007/s12010-021-03590-0
通讯作者	Chen, Xinde(cxd_cxd@hotmail.com)
英文摘要	Understanding factors that affect the catalytic efficiency and synergism of enzymes is helpful to enhance the process of bioconversion. In this study, birch wood (BW) was sequentially treated by delignification (DL), deacetylation (DA), and decrystallization (DC) treatments. The physiochemical structures of treated BW were characterized. Moreover, the influences of sequential treatments on the catalytic efficiency and synergism of xylanase and cellulase were studied. DL treatments efficiently improved the conversion of cellulose and xylan. A high degree of synergy (DS) between xylanase and cellulase was produced during hydrolysis of DL-treated BW. DA treatments enhanced xylan conversion but reduced the DS between xylanase and cellulase for xylan hydrolysis, whereas DC treatments enhanced cellulose conversion but reduced the DS between xylanase and cellulase for cellulose hydrolysis. The cellulose conversion of lithium chloride/N,N-dimethylacetamide (LiCl/DMAc)-treated BW (89.69%) was higher than the cellulose conversion of ball milling (BM)-treated BW (81.63%), whereas the xylan conversion of LiCl/DMAc-treated BW (83.77%) was lower than the xylan conversion of BM-treated BW (87.21%). This study showed that the catalytic efficiency and synergism of xylanase and cellulase are markedly affected by lignin hindrance, hemicellulose acetylation, and cellulose crystallization.
资助项目	National Natural Science Foundation of China[31600475] ; National Natural Science Foundation of China[51876207] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515011843] ; Project of Jiangsu Province Science and Technology[BE2018342] ; Project of Jiangsu Province Science and Technology[BE2020394]
WOS关键词	LIGNOCELLULOSIC BIOMASS ; FERMENTATION ; PRETREATMENT
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000672454300001
资助机构	National Natural Science Foundation of China ; Guangdong Basic and Applied Basic Research Foundation ; Project of Jiangsu Province Science and Technology
内容类型	期刊论文
源URL	[http://ir.giec.ac.cn/handle/344007/33463]
专题	中国科学院广州能源研究所
通讯作者	Chen, Xinde
作者单位	1.Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 2.CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Guangzhou Inst Energy Convers, 2 Energy Rd, Guangzhou 510640, Peoples R China 5.Guangdong Pharmaceut Univ, Sch Food Sci, Zhongshan 528458, Peoples R China 6.R&D Caner Xuyi Attapulgite Energy & Environm Mat, Xuyi 211700, Peoples R China
推荐引用方式 GB/T 7714	Li, Hailong,Chen, Xindong,Wang, Can,et al. Factors Affecting the Catalytic Efficiency and Synergism of Xylanase and Cellulase During Enzymatic Hydrolysis of Birch Wood[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2021:14.
APA	Li, Hailong.,Chen, Xindong.,Wang, Can.,Chen, Xuefang.,Guo, Haijun.,...&Chen, Xinde.(2021).Factors Affecting the Catalytic Efficiency and Synergism of Xylanase and Cellulase During Enzymatic Hydrolysis of Birch Wood.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,14.
MLA	Li, Hailong,et al."Factors Affecting the Catalytic Efficiency and Synergism of Xylanase and Cellulase During Enzymatic Hydrolysis of Birch Wood".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2021):14.