Non-enzymatic Transformation of Aflatoxin B-1 by Pseudomonas geniculata m29

doi:10.3389/fmicb.2021.724103

	Non-enzymatic Transformation of Aflatoxin B-1 by Pseudomonas geniculata m29
	Yao, Yuanyuan 1,2; Shu, Xian 1,2; Wang, Dongdong 1; Kan, Wenjie 1; Su, Pengfei 1; Hu, Hao 1; Chen, Xu 1; Wang, Dacheng 1; Huang, Shengwei 1; Wu, Lifang 1,2
刊名	FRONTIERS IN MICROBIOLOGY
	2021-08-10
卷号	12
关键词	aflatoxin B-1 Pseudomonas geniculata m29 transformation non-enzymatic LC-MS analysis
DOI	10.3389/fmicb.2021.724103
通讯作者	Huang, Shengwei(swhuang@ipp.ac.cn) ; Wu, Lifang(lfwu@ipp.ac.cn)
英文摘要	Aflatoxin B-1 (AFB(1)) is the most harmful mycotoxin produced by filamentous fungi and presents a serious threat to human and animal health. Therefore, it is essential to protect humans and animals from AFB(1)-induced acute and chronic toxicity. In this study, Pseudomonas strain m29 having a high efficiency of AFB(1) transformation was isolated from soil. The transformation ratio by m29 was more than 97% within 24 h, and the optimum temperature for transformation was 37 degrees C. Moreover, the AFB(1) transforming activity was mainly attributed to the cell-free supernatant of strain m29. The metabolite that plays a crucial role in AFB(1) transformation is likely 1,2-dimethylhydrazine or 1,1-dimethylhydrazine, as identified by GC-MS and LC-MS analysis. AFB(1) was transformed into a product with molecular formula C17H14O7. To the best of our knowledge, this is the first study of non-enzymatic AFB(1) transformation by bacteria. Importantly, this AFB(1) transformation mechanism could be universal to various microorganisms.
资助项目	Natural Science Foundation of Anhui Province[2008085MC60] ; Key Program of 13th Five-Year Plan, Hefei Institutes of Physical Science, Chinese Academy of Sciences[kp-2019-21]
WOS关键词	ASPERGILLUS-FLAVUS ; SODIUM BISULFITE ; DEGRADATION ; DETOXIFICATION ; BIODEGRADATION ; STRAIN ; DESTRUCTION ; PARASITICUS ; TEMPERATURE ; PEROXIDASE
WOS研究方向	Microbiology
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000687884700001
资助机构	Natural Science Foundation of Anhui Province ; Key Program of 13th Five-Year Plan, Hefei Institutes of Physical Science, Chinese Academy of Sciences
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/125123]
专题	中国科学院合肥物质科学研究院
通讯作者	Huang, Shengwei; Wu, Lifang
作者单位	1.Chinese Acad Sci, Hefei Inst Phys Sci, Key Lab High Magnet Field & Ion Beam Phys Biol, Hefei, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei, Peoples R China
推荐引用方式 GB/T 7714	Yao, Yuanyuan,Shu, Xian,Wang, Dongdong,et al. Non-enzymatic Transformation of Aflatoxin B-1 by Pseudomonas geniculata m29[J]. FRONTIERS IN MICROBIOLOGY,2021,12.
APA	Yao, Yuanyuan.,Shu, Xian.,Wang, Dongdong.,Kan, Wenjie.,Su, Pengfei.,...&Wu, Lifang.(2021).Non-enzymatic Transformation of Aflatoxin B-1 by Pseudomonas geniculata m29.FRONTIERS IN MICROBIOLOGY,12.
MLA	Yao, Yuanyuan,et al."Non-enzymatic Transformation of Aflatoxin B-1 by Pseudomonas geniculata m29".FRONTIERS IN MICROBIOLOGY 12(2021).