The Role of Apoptosis Pathway in the Cytotoxicity Induced by Fresh and Aged Zinc Oxide Nanoparticles

doi:10.1186/s11671-021-03587-y

	The Role of Apoptosis Pathway in the Cytotoxicity Induced by Fresh and Aged Zinc Oxide Nanoparticles
	Wang,Juan 2,3; Wang,Lei 1; Zhao,Wenting 2; Yu,Na 2; Cheng,Meiling 2; Su,Mingqin 2; Hu,Jian 2; Wu,Xiaoyan 3; Du,Hua 4; Wang,Meimei 2,3
刊名	Nanoscale Research Letters
	2021-08-09
卷号	16
关键词	Aged zinc oxide nanoparticles Cytotoxicity Mechanism Apoptosis Transcriptomics
ISSN号	1931-7573
DOI	10.1186/s11671-021-03587-y
通讯作者	Du,Hua(huadu@ipp.ac.cn) ; Wang,Meimei(wangmm@ustc.edu.cn)
英文摘要	AbstractZinc oxide nanoparticles (ZnO NPs) are used in a wide range of applications including industry, commercial products and medicine field. Numerous mechanistic studies for ZnO NPs’ toxicity were performed on pristine (fresh) NPs. However, the cytotoxicity induced by the transformed (aged) ZnO NPs and the underlying mechanisms remain unclear. Here, we observed the physicochemical transformation of ZnO NPs underwent over time, followed by evaluating the cytotoxicity of fresh and aged NPs. We found that fresh ZnO NPs induced higher apoptosis level than their aged counterparts. Accordingly, RNA sequencing data from aged ZnO NP-treated human–hamster hybrid (AL) cells showed that p53, PI3k–Akt, FoXO, Glutathione, ErbB, HIF-1, Oxytocin and Jak-STAT signaling pathways were enriched but no apoptosis pathway. Quantitative PCR results revealed the significantly higher mRNA level of IL1B and CD69 in fresh NP-treated groups compared to that of aged ZnO NP- and zinc chloride-treated groups. The above results indicated that the lower cytotoxicity of aged ZnO NPs is partially attributed to their reduced potency in inducing apoptosis. The transcriptional regulation of multiple signal pathways activated by aged NPs may help to build the cellular homeostasis. Taken together, our findings highlight the influence of aging (environmental transformation) process of ZnO NPs on their toxicities and biological consequences.
资助项目	National Natural Science Foundation of China[22006149] ; National Natural Science Foundation of China[21507002] ; National Natural Science Foundation of China[21677147] ; Anhui Provincial Natural Science Foundation[2008085MB49] ; Hundred Talents Program of the Chinese Academy of Sciences[E09BR22] ; Open Project Fund of the Key Laboratory of the Ministry of Education[JKZD20202] ; National undergraduate innovation and entrepreneurship training program[202010366043]
WOS关键词	PULSED-LASER ABLATION ; IN-VITRO CYTOTOXICITY ; PHYSICOCHEMICAL TRANSFORMATIONS ; CUO NANOPARTICLES ; BLACK TIO2 ; ACTIVATION ; MECHANISM ; TOXICITY ; CELLS ; ZNO
WOS研究方向	Science & Technology - Other Topics ; Materials Science ; Physics
语种	英语
出版者	Springer US
WOS记录号	BMC:10.1186/S11671-021-03587-Y
资助机构	National Natural Science Foundation of China ; Anhui Provincial Natural Science Foundation ; Hundred Talents Program of the Chinese Academy of Sciences ; Open Project Fund of the Key Laboratory of the Ministry of Education ; National undergraduate innovation and entrepreneurship training program
内容类型	期刊论文
源URL	[http://ir.hfcas.ac.cn:8080/handle/334002/124005]
专题	中国科学院合肥物质科学研究院
通讯作者	Du,Hua; Wang,Meimei
作者单位	1.University of Miami; Department of Physiology and Biophysics, Miller School of Medicine 2.Anhui Medical University; Department of Pathophysiology, School of Basic Medical Science 3.MOE Key Laboratory of Population Health Across Life Cycle 4.Chinese Academy of Sciences; High Magnetic Field Laboratory, Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science
推荐引用方式 GB/T 7714	Wang,Juan,Wang,Lei,Zhao,Wenting,et al. The Role of Apoptosis Pathway in the Cytotoxicity Induced by Fresh and Aged Zinc Oxide Nanoparticles[J]. Nanoscale Research Letters,2021,16.
APA	Wang,Juan.,Wang,Lei.,Zhao,Wenting.,Yu,Na.,Cheng,Meiling.,...&Wang,Meimei.(2021).The Role of Apoptosis Pathway in the Cytotoxicity Induced by Fresh and Aged Zinc Oxide Nanoparticles.Nanoscale Research Letters,16.
MLA	Wang,Juan,et al."The Role of Apoptosis Pathway in the Cytotoxicity Induced by Fresh and Aged Zinc Oxide Nanoparticles".Nanoscale Research Letters 16(2021).