Measurement of the interconnected turgor pressure and envelope elasticity of live bacterial cells

doi:10.1039/d0sm02075c

	Measurement of the interconnected turgor pressure and envelope elasticity of live bacterial cells
	Zhang, Huanxin 2; Wang, Huabin 3,4,5; Wilksch, Jonathan J.6,7; Strugnell, Richard A.1; Gee, Michelle L.8; Feng, Xi-Qiao 2
刊名	SOFT MATTER
	2021-02-28
卷号	17 期号:8 页码:2042-2049
ISSN号	1744-683X
DOI	10.1039/d0sm02075c
通讯作者	Wang, Huabin(wanghuabin@cigit.ca.cn) ; Feng, Xi-Qiao(fengxq@tsinghua.edu.cn)
英文摘要	Turgor pressure and envelope elasticity of bacterial cells are two mechanical parameters that play a dominant role in cellular deformation, division, and motility. However, a clear understanding of these two properties is lacking because of their strongly interconnected mechanisms. This study established a nanoindentation method to precisely measure the turgor pressure and envelope elasticity of live bacteria. The indentation force-depth curves of Klebsiella pneumoniae bacteria were recorded with atomic force microscopy. Through combination of dimensional analysis and numerical simulations, an explicit expression was derived to decouple the two properties of individual bacteria from the nanoindentation curves. We show that the Young's modulus of bacterial envelope is sensitive to the external osmotic environment, and the turgor pressure is significantly dependent on the external osmotic stress. This method can not only quantify the turgor pressure and envelope elasticity of bacteria, but also help resolve the mechanical behaviors of bacteria in different environments.
资助项目	National Key R&D Program of China[2017YFF0106303] ; National Natural Science Foundation of China[U1932132] ; National Natural Science Foundation of China[11921002] ; National Natural Science Foundation of China[11620101001] ; Natural Science Foundation of Chongqing[cstc2018jcyjAX0405] ; Chongqing Innovation and Entrepreneurship Program for Excellent Returned Overseas Chinese Talents[Y82z440] ; Australian Government National Health and Medical Research Council Program Grant[1092262] ; Australian Research Council[DP170104321]
WOS研究方向	Chemistry ; Materials Science ; Physics ; Polymer Science
语种	英语
出版者	ROYAL SOC CHEMISTRY
WOS记录号	WOS:000625282600030
内容类型	期刊论文
源URL	[http://119.78.100.138/handle/2HOD01W0/13209]
专题	中国科学院重庆绿色智能技术研究院
通讯作者	Wang, Huabin; Feng, Xi-Qiao
作者单位	1.Univ Melbourne, Dept Microbiol & Immunol, Parkville, Vic 3010, Australia 2.Tsinghua Univ, Inst Biomech & Med Engn, Dept Engn Mech, AML, Beijing 100084, Peoples R China 3.Chinese Acad Sci, Chongqing Inst Green & Intelligent Technol, Res Ctr Appl Phys, Chongqing 400714, Peoples R China 4.Univ Chinese Acad Sci, Chongqing Sch, Chongqing 400714, Peoples R China 5.Chongqing Engn Res Ctr High Resolut & Three Dimen, Chongqing 400714, Peoples R China 6.Monash Univ, Biomed Discovery Inst, Infect & Immun Program, Clayton, Vic 3800, Australia 7.Monash Univ, Dept Microbiol, Clayton, Vic 3800, Australia 8.RMIT Univ, Sch Aerosp Engn & Aviat, Bundoora, Vic 3083, Australia
推荐引用方式 GB/T 7714	Zhang, Huanxin,Wang, Huabin,Wilksch, Jonathan J.,et al. Measurement of the interconnected turgor pressure and envelope elasticity of live bacterial cells[J]. SOFT MATTER,2021,17(8):2042-2049.
APA	Zhang, Huanxin,Wang, Huabin,Wilksch, Jonathan J.,Strugnell, Richard A.,Gee, Michelle L.,&Feng, Xi-Qiao.(2021).Measurement of the interconnected turgor pressure and envelope elasticity of live bacterial cells.SOFT MATTER,17(8),2042-2049.
MLA	Zhang, Huanxin,et al."Measurement of the interconnected turgor pressure and envelope elasticity of live bacterial cells".SOFT MATTER 17.8(2021):2042-2049.