Association between the polymorphism of CfPGRP-S gene and disease susceptibility/resistance of zhikong scallop (Chlamys farreri) to Listonella anguillarum challenge

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	Association between the polymorphism of CfPGRP-S gene and disease susceptibility/resistance of zhikong scallop (Chlamys farreri) to Listonella anguillarum challenge
	Siva, Vinu S.1,2; Yang, Chuanyan 1,2; Yang, Jialong 1,2; Wang, Lingling 1; Wang, Leilei 1,2; Zhou, Zhi 1,2; Qiu, Limei 1; Song, Linsheng 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2012-10-01
卷号	33 期号:4 页码:736-742
关键词	Zhikong scallop Chlamys farreri Peptidoglycan recognition protein Single nucleotide polymorphism Pathogen-associated molecular patterns (PAMP) binding Antimicrobial activity
ISSN号	1050-4648
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China.
英文摘要	Peptidoglycan recognition protein (PGRP) is a pattern recognition receptor, playing important roles in the innate immune response against invasive pathogens. The single nucleotide polymorphism (SNP) loci in scallop PGRP gene (CfPGRP) were screened from Chlamys farreri to investigate their association with disease resistance of scallop against Listonella anguillarum. Thirteen SNP sites were identified in PGRP domain of CfPGRP, and two of them at positions 4407 and 4408 which are located in the same codon resulted in a nonsynonymous substitution. The genotype frequency of CG/CG in the resistant stock was significantly lower than that in susceptible stock (0% vs 32.4%), while that of CG/TA in the resistant stock was significantly higher than that in susceptible stock (P < 0.01). The pathogen-associated molecular patterns (PAMP) binding activity of two recombinant proteins, rCfPGRP-S1 (R) with CG variant in 4407 -4408 site, rCfPGRP-S1 (Y) with TA variant in 4407-4408 site, were elucidated by examining their P/N value at 405 nm with ELISA assay. The in vitro binding activities of the two rCfPGRP-S1 variants to both lipopolysaccharide (LPS) and peptidoglycan (PGN) varied (P < 0.05) in a dose-dependent manner, and rCfPRPP-S1(Y) exhibited significantly higher affinity to PGN and LPS than that of rCfPGRP-S1(R) (P < 0.05). The growth inhibition assay was conducted to find the antibacterial activities of the two variants. Both rCfPGRP-S1(R) and rCfPGRP-S1 (Y) displayed obvious activity to suppress the growth of Escherichia coli, but there was no significant difference in suppression activity of two variants (P > 0.05). The results suggested that the polymorphism at locus 4407-4408 of CfPGRP-S1 considerably affected its PAMP binding activity, and the SNP locus 4407-4408 CG/TA was associated with disease resistance of scallop against L anguillarum infection, which could be used as a candidate marker for future selection in zhikong scallop breeding program. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
收录类别	SCI
原文出处	10.1016/j.fsi.2012.06.016
语种	英语
WOS记录号	WOS:000310185800008
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/12287]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Siva, Vinu S.,Yang, Chuanyan,Yang, Jialong,et al. Association between the polymorphism of CfPGRP-S gene and disease susceptibility/resistance of zhikong scallop (Chlamys farreri) to Listonella anguillarum challenge[J]. FISH & SHELLFISH IMMUNOLOGY,2012,33(4):736-742.
APA	Siva, Vinu S..,Yang, Chuanyan.,Yang, Jialong.,Wang, Lingling.,Wang, Leilei.,...&Song, Linsheng.(2012).Association between the polymorphism of CfPGRP-S gene and disease susceptibility/resistance of zhikong scallop (Chlamys farreri) to Listonella anguillarum challenge.FISH & SHELLFISH IMMUNOLOGY,33(4),736-742.
MLA	Siva, Vinu S.,et al."Association between the polymorphism of CfPGRP-S gene and disease susceptibility/resistance of zhikong scallop (Chlamys farreri) to Listonella anguillarum challenge".FISH & SHELLFISH IMMUNOLOGY 33.4(2012):736-742.