Late Devonian ostracods are described for the first time from the Frasnian-Famennian (F-F) transition of the Zengpiyan section, Guangxi, South China. Forty-five ostracod species belonging to 25 genera are identified and figured and one is new: Jenningsina guilinensis sp. nov. The Frasnian-Famennian boundary (F-FB) in the Zengpiyan section is coincident with the lithological boundary between the Guilin Formation and the Dongcun Formation, and could be marked by the disappearance of Rectobairdia proximischimensis (Lethiers & Casiers, 1998) and Jenningsina guilinensis sp. nov. The ostracods belong to the Eifelian Mega-Assemblage, which implies a shallow-water palaeoenvironment. The ostracod fauna and sedimentary features of the Guilin and Dongcun formations at the Zengpiyan section suggest a transition from subtidal to low-energy tidal flat on the Guilin Platform during the Frasnian-Famennian event. The extinction rate of benthic ostracod species is about 61% during the F-F event in the Zengpiyan section. Two stages of faunal changes have been recognized in the event and the second stage is more severe than the first one.