Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng | |
Zuo, Tiantian1,2; Zhang, Chunxia1,2; Li, Weiwei1,2; Wang, Hongda1,2; Hu, Ying1,2; Yang, Wenzhi1,2; Jia, Li1,2; Wang, Xiaoyan1,2; Gao, Xiumei1; Guo, Dean1,3 | |
刊名 | JOURNAL OF PHARMACEUTICAL ANALYSIS |
2020-12-01 | |
卷号 | 10期号:6页码:597-609 |
关键词 | Dimension-enhanced strategy Multicomponent characterization Ginsenoside Offline two-dimensional liquid chromatography Ion mobility-quadrupole time-of-flight mass spectrometry In-house database |
ISSN号 | 2095-1779 |
DOI | 10.1016/j.jpha.2019.11.001 |
通讯作者 | Yang, Wenzhi(wzyang0504@tjutcm.edu.cn) ; Guo, Dean(daguo@simm.ac.cn) |
英文摘要 | Inherent complexity of plant metabolites necessitates the use of multi-dimensional information to accomplish comprehensive profiling and confirmative identification. A dimension-enhanced strategy, by offline two-dimensional liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (2D-LC/IM-QTOE-MS) enabling four-dimensional separations (2D-LC, IM, and MS), is proposed. In combination with in-house database-driven automated peak annotation, this strategy was utilized to characterize ginsenosides simultaneously from white ginseng (WG) and red ginseng (RG). An offline 2D-LC system configuring an Xbridge Amide column and an HSS T3 column showed orthogonality 0.76 in the resolution of ginsenosides. Ginsenoside analysis was performed by data-independent high-definition MSE(HDMSE) in the negative ESI mode on a Vion (TM) IMS-QTOF hybrid high-resolution mass spectrometer, which could better resolve ginsenosides than MSE and directly give the CCS information. An in-house ginsenoside database recording 504 known ginsenosides and 58 reference compounds, was established to assist the identification of ginsenosides. Streamlined workflows, by applying UNIFI (TM) to automatedly annotate the HDMSE data, were proposed. We could separate and characterize 323 ginsenosides (including 286 from WG and 306 from RG), and 125 thereof may have not been isolated from the Panax genus. The established 2D-LC/IM-QTOE-HDMSE approach could also act as a magnifier to probe differentiated components between WG and RG. Compared with conventional approaches, this dimension-enhanced strategy could better resolve coeluting herbal components and more efficiently, more reliably identify the multicomponents, which, we believe, offers more possibilities for the systematic exposure and confirmative identification of plant metabolites. (C) 2019 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. |
资助项目 | National Natural Science Foundation of China[81872996] ; State Key Research and Development Project[2017YFC1702104] ; State Key Project for the Creation of Major New Drugs[2018ZX09711001009-010] ; Tianjin Municipal Education Commission Research Project[2017ZD07] |
WOS关键词 | COLLISION CROSS-SECTION ; PANAX-NOTOGINSENG ; COMPREHENSIVE CHARACTERIZATION ; DATA-ACQUISITION ; SAPONINS ; IDENTIFICATION ; GINSENOSIDES ; DISCOVERY ; METABOLITES ; ALKALOIDS |
WOS研究方向 | Pharmacology & Pharmacy |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000604958600010 |
内容类型 | 期刊论文 |
源URL | [http://119.78.100.183/handle/2S10ELR8/296043] |
专题 | 中国科学院上海药物研究所 |
通讯作者 | Yang, Wenzhi; Guo, Dean |
作者单位 | 1.Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, 312 Anshanxi Rd, Tianjin 300193, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Tianjin Key Lab TCM Chem & Anal, 312 Anshanxi Rd, Tianjin 300193, Peoples R China 3.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China |
推荐引用方式 GB/T 7714 | Zuo, Tiantian,Zhang, Chunxia,Li, Weiwei,et al. Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng[J]. JOURNAL OF PHARMACEUTICAL ANALYSIS,2020,10(6):597-609. |
APA | Zuo, Tiantian.,Zhang, Chunxia.,Li, Weiwei.,Wang, Hongda.,Hu, Ying.,...&Guo, Dean.(2020).Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng.JOURNAL OF PHARMACEUTICAL ANALYSIS,10(6),597-609. |
MLA | Zuo, Tiantian,et al."Offline two-dimensional liquid chromatography coupled with ion mobility-quadrupole time-of-flight mass spectrometry enabling four-dimensional separation and characterization of the multicomponents from white ginseng and red ginseng".JOURNAL OF PHARMACEUTICAL ANALYSIS 10.6(2020):597-609. |
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