Characterization of anti-BCG benz[alpha]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomyceteNocardiasp. XJ31

doi:10.1007/s00253-020-10842-2

	Characterization of anti-BCG benz[alpha]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomyceteNocardiasp. XJ31
	Zhang, Li 1,2,3; Zhang, Jingyu 1; Ren, Biao 4,5; Lu, Wanying 1; Hou, Chengjian 1; Wang, Jian 2; Ma, Xiaolong 2; Ma, Rong 1; Liu, Mei 2; Liu, Zhiheng 2
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2020-10-01
卷号	104 期号:19 页码:8267-8278
关键词	Xinjiang desert Nocardiasp Anti-BCG Brasiliquinone Nocardimicin
ISSN号	0175-7598
DOI	10.1007/s00253-020-10842-2
通讯作者	Dai, Huanqin(daihq@im.ac.cn) ; Liu, Xueting(liuxueting@ecust.edu.cn)
英文摘要	The current global demand for novel anti-TB drugs has drawn urgent attention on the discovery of natural product compounds with anti-TB activity. Lots of efforts have emphasized on environmental samples from unexplored or underexplored natural habits and identified numerous rare actinomycete taxa producing structurally diverse bioactive natural products. Herein, we report a survey of the rare actinobacteria diversity in Xinjiang region together with the discovery of anti-TB active natural products from these strains. We have collected 17 soil samples at different sites with different environmental conditions, from which 39 rare actinobacteria were identified by using a selective isolation strategy with 5 media variations. Among those isolated strains, XJ31 was identified as a newNocardiasp. based on 16S rRNA gene analysis. Through one strain-many compounds (OSMAC) strategy combined with anti-Bacillus Calmette-Guerin bioassay-guided isolation, two groups of compounds were identified. They were twelve siderophores (nocardimicins,1-12) and two anthraquinones (brasiliquinones,13and14) and ten of them were identified as new compounds. The structures of the purified compounds were elucidated using HR-ESI-MS, 1D NMR, and 2D NMR techniques. The anti-TB bioassays revealed that the two benz[alpha]anthraquinones have potent activity against BCG (MICs = 25 mu M), which can be used as a promising start point for further anti-TB drug development.
资助项目	National Key Research and Development Program of China[2019YFA0906200] ; National Natural Science Foundation of China[31720103901] ; National Natural Science Foundation of China[21877038] ; National Natural Science Foundation of China[21977029] ; National Natural Science Foundation of China[21877124] ; National Natural Science Foundation of China[81573341] ; Shanghai Rising-Star Program[20QA1402800] ; Open Project Funding of the State Key Laboratory of Bioreactor Engineering ; 111 Project[B18022] ; Shanghai Science and Technology Commission[18JC1411900]
WOS关键词	NATURAL-PRODUCTS ; SP NOV. ; A-C ; IRON ; DIVERSITY ; SOIL ; METABOLITES ; DISCOVERY ; SEDIMENT ; AGENTS
WOS研究方向	Biotechnology & Applied Microbiology
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000562287500004
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/292456]
专题	中国科学院上海药物研究所
通讯作者	Dai, Huanqin; Liu, Xueting
作者单位	1.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol SKLM, Beijing 100101, Peoples R China 3.Boston Univ, Dept Chem, 590 Commonwealth Ave, Boston, MA 02215 USA 4.Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610041, Peoples R China 5.Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Chengdu 610041, Peoples R China 6.Uppsala Univ, Biomed Ctr, Dept Med Biochem & Microbiol, SciLifeLab Uppsala, Husatgatan 3, S-75123 Uppsala, Sweden 7.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Key Lab Receptor Res, Shanghai 201203, Peoples R China 8.Univ Chinese Acad Sci, 19 A Yuquan Rd, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Li,Zhang, Jingyu,Ren, Biao,et al. Characterization of anti-BCG benz[alpha]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomyceteNocardiasp. XJ31[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2020,104(19):8267-8278.
APA	Zhang, Li.,Zhang, Jingyu.,Ren, Biao.,Lu, Wanying.,Hou, Chengjian.,...&Liu, Xueting.(2020).Characterization of anti-BCG benz[alpha]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomyceteNocardiasp. XJ31.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,104(19),8267-8278.
MLA	Zhang, Li,et al."Characterization of anti-BCG benz[alpha]anthraquinones and new siderophores from a Xinjiang desert-isolated rare actinomyceteNocardiasp. XJ31".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 104.19(2020):8267-8278.