Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values

doi:10.1016/j.jare.2020.04.011

	Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values
	Huo, Jinxi 2; Zhong, Shi 2; Du, Xin 2; Cao, Yinglong 1,3; Wang, Wenqiong 1,3; Sun, Yuqing 2; Tian, Yu 4; Zhu, Jianxun 2; Chen, Jine 2; Xuan, Lijiang 1,3
刊名	JOURNAL OF ADVANCED RESEARCH
	2020-07-01
卷号	24 页码:325-335
关键词	Anti-carcinogenesis activity Genome sequencing HR-ESI-MS Inoscavin A Phellinus gilvus Phenylpropanoids biosynthesis
ISSN号	2090-1232
DOI	10.1016/j.jare.2020.04.011
通讯作者	Wu, Chongming() ; Li, Yougui()
英文摘要	Phellinus gilvus (Schwein.) Pat, a species of 'Sanghuang', has been well-documented for various medicinal uses, but the genome information and active constituents are largely unknown. Here, we sequenced the whole-genome of P. gilvus, identified phenylpropanoids as its key anti-cancer components, and deduced their biosynthesis pathways. A 41.11-Mb genome sequence was assembled and the heatmap created with high-throughput chromosome conformation capture techniques data suggested all bins could be clearly divided into 11 pseudochromosomes. Cellular experiments showed that P. gilvus fruiting body was more effective to inhibit hepatocellular carcinoma cells than mycelia. High resolution electrospray ionization mass spectroscopy (HR-ESI-MS) analysis revealed P. gilvus fruiting body was rich in phenylpropanoids, and several unique phenylpropanoids in Phellinus spp. exhibited potent anti-carcinogenesis activity. Based on genomic, HR-ESI-MS information and differentially expressed genes in transcriptome analysis, we deduced the biosynthesis pathway of four major phenylpropanoids in P. gilvus. Transcriptome analysis revealed the deduced genes expressions were synergistically changed with the production of phenylpropanoids. The optimal candidate genes of phenylpropanoids' synthesis pathway were screened by molecular docking analysis. Overall, our results provided a high-quality genomic data of P. gilvus and inferred biosynthesis pathways of four phenylpropanoids with potent anti-carcinogenesis activities. These will be a valuable resource for further genetic improvement and effective use of the P. gilvus. (C) 2020 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
资助项目	Science and Technology Department of Zhejiang Province[2018C02003]
WOS关键词	HI-C ; HISPIDIN BIOSYNTHESIS ; IDENTIFICATION ; DATABASE ; INONOTUS ; FAMILIES ; CLASSIFICATION ; INHIBITION ; ASSEMBLIES ; ALIGNMENT
WOS研究方向	Science & Technology - Other Topics
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000550190000003
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291986]
专题	中国科学院上海药物研究所
通讯作者	Wu, Chongming; Li, Yougui
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Zhejiang Acad Agr Sci, Sericultural Res Inst, Hangzhou 310021, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Pharmacol & Toxicol Res Ctr, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Huo, Jinxi,Zhong, Shi,Du, Xin,et al. Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values[J]. JOURNAL OF ADVANCED RESEARCH,2020,24:325-335.
APA	Huo, Jinxi.,Zhong, Shi.,Du, Xin.,Cao, Yinglong.,Wang, Wenqiong.,...&Li, Yougui.(2020).Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values.JOURNAL OF ADVANCED RESEARCH,24,325-335.
MLA	Huo, Jinxi,et al."Whole-genome sequence of Phellinus gilvus (mulberry Sanghuang) reveals its unique medicinal values".JOURNAL OF ADVANCED RESEARCH 24(2020):325-335.