Baicalin Inhibits Influenza A Virus InfectionviaPromotion of M1 Macrophage Polarization

doi:10.3389/fphar.2020.01298

	Baicalin Inhibits Influenza A Virus InfectionviaPromotion of M1 Macrophage Polarization
	Geng, Ping 2,3; Zhu, Haiyan 2,3; Zhou, Wei 4; Su, Chang 1; Chen, Mingcang 5; Huang, Chenggang 5; Xia, Chengjie 2,3; Huang, Hai 2,3; Cao, Yiou 1; Shi, Xunlong 2,3
刊名	FRONTIERS IN PHARMACOLOGY
	2020-10-06
卷号	11 页码:10
关键词	macrophage M1 polarization influenza virus baicalin metabolism
ISSN号	1663-9812
DOI	10.3389/fphar.2020.01298
通讯作者	Cao, Yiou(yioucao_doctor@126.com) ; Shi, Xunlong(xunlongshi@fudan.edu.cn)
英文摘要	Background and Aims The natural compound baicalin (BA) possesses potent antiviral properties against the influenza virus. However, the underlying molecular mechanisms of this antiviral activity and whether macrophages are involved remain unclear. In this study, we, therefore, investigated the effect of BA on macrophages. Methods We studied macrophage recruitment, functional phenotypes (M1/M2), and the cellular metabolismviaflow cytometry, qRT-PCR, immunofluorescence, a cell culture transwell system, and GC-MS-based metabolomics bothin vivoin H1N1 A virus-infected mice andin vitro. Results BA treatment drastically reduced macrophage recruitment (CD11b(+), F4/80(+)) by approximately 90% while maintaining the proportion of M1-polarized macrophages in the bronchoalveolar lavage fluid of infected mice. This BA-stimulated macrophage M1 phenotype shift was further verifiedin vitroin ANA-1 and primary peritoneal macrophages by measuring macrophage M1 polarization signals (CD86, iNOS, TNF-alpha,iNOS/Arg-1ratio, and IL-1 beta cleavage). Meanwhile, we observed an activation of the IFN pathway (upregulation ofIFN-beta andIRF-3), an inhibition of influenza virus replication (as measured by theMgene), and distinct cellular metabolic responses in BA-treated cells. Conclusion BA triggered macrophage M1 polarization, IFN activation, and other cellular reactions, which are beneficial for inhibition of H1N1 A virus infection.
资助项目	Nation Nature Science Foundation of China[U1604283] ; Shanghai Science and Technology Funds[17ZR1401700] ; Shanghai Municipal Health Commission project[201740200]
WOS关键词	ANTIVIRAL ACTIVITY ; METABOLOMICS ; ACTIVATION ; INFECTION ; METABOLISM
WOS研究方向	Pharmacology & Pharmacy
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000579553700001
内容类型	期刊论文
源URL	[http://119.78.100.183/handle/2S10ELR8/291438]
专题	中国科学院上海药物研究所
通讯作者	Cao, Yiou; Shi, Xunlong
作者单位	1.Fudan Univ, Minhang Hosp, Dept Surg, Shanghai, Peoples R China 2.Fudan Univ, Sch Pharm, Dept Biol Med, Shanghai, Peoples R China 3.Fudan Univ, Sch Pharm, Shanghai Engn Res Ctr Immunotherapeut, Shanghai, Peoples R China 4.Fudan Univ, Dept Chem, Shanghai, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Geng, Ping,Zhu, Haiyan,Zhou, Wei,et al. Baicalin Inhibits Influenza A Virus InfectionviaPromotion of M1 Macrophage Polarization[J]. FRONTIERS IN PHARMACOLOGY,2020,11:10.
APA	Geng, Ping.,Zhu, Haiyan.,Zhou, Wei.,Su, Chang.,Chen, Mingcang.,...&Shi, Xunlong.(2020).Baicalin Inhibits Influenza A Virus InfectionviaPromotion of M1 Macrophage Polarization.FRONTIERS IN PHARMACOLOGY,11,10.
MLA	Geng, Ping,et al."Baicalin Inhibits Influenza A Virus InfectionviaPromotion of M1 Macrophage Polarization".FRONTIERS IN PHARMACOLOGY 11(2020):10.